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5.4.99.30: UDP-arabinopyranose mutase

This is an abbreviated version!
For detailed information about UDP-arabinopyranose mutase, go to the full flat file.

Word Map on EC 5.4.99.30

Reaction

UDP-beta-L-arabinofuranose
=
UDP-beta-L-arabinopyranose

Synonyms

AtRGP1, AtRGP2, AtRGP3, BdRGP1, Bradi1g15050, Bradi1g21990, Bradi2g50660, Bradi5g24850, CrUAM, LOC100822188, LOC100828998, LOC100836426, LOC100838265, NtUAM, Os03g0599800 protein, Os03g40270 protein, Os07g0604800 protein, Os07g41360 protein, OsUAM, OsUAM1, OsUAM3, RGP1, RGP2, RGP3, RGP4, RGPs3, UAM, UAM1, UAM3, UAMs3, UDP-arabinopyranose mutase, UDP-arabinopyranose mutase 3

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.30 UDP-arabinopyranose mutase

Purification

Purification on EC 5.4.99.30 - UDP-arabinopyranose mutase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified 94fold using hydrophobic chromatography and gel filtration
-
recombinant His-MBP-tagged isozyme RGP1 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration
recombinant His-MBP-tagged isozyme RGP2 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration
recombinant His-MBP-tagged isozyme RGP3 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration
recombinant His-tagged wild-type and mutant isozymes UAM1 from Escherichia coli strain BL21 by nickel affinity chromatography