5.4.99.27: tRNA pseudouridine13 synthase

This is an abbreviated version!
For detailed information about tRNA pseudouridine13 synthase, go to the full flat file.

Reaction

Synonyms

Pab aPus7, PSI, PUS7, Pus7 protein, Pus7p, RNA:pseudouridine-synthase Pus7p, RNA:PSI-synthase Pus7p, Sso aPus7, tRNA PSI13 synthase, TruD, YgbO

ECTree

     5 Isomerases

         5.4 Intramolecular transferases

             5.4.99 Transferring other groups

                5.4.99.27 tRNA pseudouridine13 synthase

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Results	in table
10684	AA Sequence
2	CAS Registry Number
7	Cloned(Commentary)
4	Crystallization (Commentary)
9	General Information
1	kcat/KM [mM/s]
1	KM Value [mM]
1	Metals/Ions
1	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
13	Organism
1	PDB ID
3	pH Optimum
18	Protein Variants
6	Purification (Commentary)
2	Reaction
13	Reference
2	Source Tissue
13	Substrates and Products (Substrate)
1	Subunits
20	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Turnover Number [1/s]

Natural Substrates Products

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Natural Substrates Products on EC 5.4.99.27 - tRNA pseudouridine13 synthase

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

Pyrococcus abyssi tRNATyr(GUA) uridine35

Pyrococcus abyssi tRNATyr(GUA) pseudouridine35

Pyrococcus abyssi

-

residue 35 reinforces the stability of the anticodon stem–loop by interaction with residue U33 (13). This structural stabilization may increase the aminoacylation efficiency of tRNATyr(GUA) by its cognate aminoacyl-tRNA synthetase

694463

-

-

?

tRNA uridine13

tRNA pseudouridine13

Saccharomyces cerevisiae

Q08647

-

747473

-

-

?

additional information

?

-

Saccharomyces cerevisiae

Q08647

for tRNA and Pus7, many known sites and their associated modifiers are identified, including Pus7-dependent sites at position 13 of glutamate tRNA. Pseudouridylation in ncRNAs at sites of inter or intra-molecular interactions

747473

-

-

?