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5.4.99.27: tRNA pseudouridine13 synthase

This is an abbreviated version!
For detailed information about tRNA pseudouridine13 synthase, go to the full flat file.

Word Map on EC 5.4.99.27

Reaction

tRNA uridine13
=
tRNA pseudouridine13

Synonyms

Pab aPus7, PSI, PUS7, Pus7 protein, Pus7p, RNA:pseudouridine-synthase Pus7p, RNA:PSI-synthase Pus7p, Sso aPus7, tRNA PSI13 synthase, TruD, YgbO

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.27 tRNA pseudouridine13 synthase

Engineering

Engineering on EC 5.4.99.27 - tRNA pseudouridine13 synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E31D
27% of wild-type activity
E31Q
no activity
E31Q/F131Y
no activity
F131Y
68% of wild-type activity
K79L/F131Y
no activity
K79L/N129K/F131Y
no activity
K79R
10% of wild-type activity
N129K
no activity
Q87E
29% of wild-type activity
K19I
-
mutation decreases the activity at position 13 in the Pyrococcus abyssi tRNAAsp at 55°C. At 80°C the mutation has almost no negative effect on its activity towards the Pyrococcus abyssi tRNATyr(GUA)
K19I/R78A/H79N
-
mutation completely abolishes the activity at position 13 in the Pyrococcus abyssi tRNAAsp at 55°C. At 80°C the mutation nearly abolishes activity towards the Pyrococcus abyssi tRNATyr(GUA)
R78A/H79N
-
mutation decreases the activity at position 13 in the Pyrococcus abyssi tRNAAsp at 55°C. At 80°C the mutation has almost no negative effect on its activity towards the Pyrococcus abyssitRNATyr(GUA)
A78R/N79H
-
mutation has no positive effect on activity at 80°C on Pyrococcus abyssi tRNAAsp(GUA)
I27K
-
mutation increases activity at 80°C on Pyrococcus abyssi tRNAAsp(GUA) 1.6fold
N79H
-
mutation has no positive effect on activity at 80°C on Pyrococcus abyssi tRNAAsp(GUA)
additional information
generation of PUS7-knockout human stem cells, hESCs, using CRISPR/Cas9 technique. There are no differences in cell proliferation, viability, and expression of multiple pluripotency, and germ layer specific markers between wild-type and PUS7-KO cells maintained in culture over long periods of time without loss of self-renewal, but a significant increase in PUS7-KO cell size compared to controls is observed. Knockout of PUS7 in HEK-293T cells