5.4.99.27: tRNA pseudouridine13 synthase

This is an abbreviated version!
For detailed information about tRNA pseudouridine13 synthase, go to the full flat file.

Reaction

Synonyms

Pab aPus7, PSI, PUS7, Pus7 protein, Pus7p, RNA:pseudouridine-synthase Pus7p, RNA:PSI-synthase Pus7p, Sso aPus7, tRNA PSI13 synthase, TruD, YgbO

ECTree

     5 Isomerases

         5.4 Intramolecular transferases

             5.4.99 Transferring other groups

                5.4.99.27 tRNA pseudouridine13 synthase

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Results	in table
10684	AA Sequence
2	CAS Registry Number
7	Cloned(Commentary)
4	Crystallization (Commentary)
9	General Information
1	kcat/KM [mM/s]
1	KM Value [mM]
1	Metals/Ions
1	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
13	Organism
1	PDB ID
3	pH Optimum
18	Protein Variants
6	Purification (Commentary)
2	Reaction
13	Reference
2	Source Tissue
13	Substrates and Products (Substrate)
1	Subunits
20	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Turnover Number [1/s]

Crystallization

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Crystallization on EC 5.4.99.27 - tRNA pseudouridine13 synthase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

crystal structure of Escherichia coli TruD at 2.0 A resolution. The structure reveals an overall V-shaped molecule with an RNA binding cleft formed between two domains: a catalytic domain and an insertion domain

Escherichia coli

Q57261

661733

crystals of TruD are grown at room temperature by the hanging drop vapor diffusion method. TruD folds into a V-shaped molecule with a catalytic domain that is structurally very similar to the catalytic modules of the other known pseudouridine synthases despite its lack of sequence homology and likely arose by divergent evolution

Escherichia coli

Q57261

652531

hanging drop vapor diffusion method, 2.2 A resolution structure. TruD reveals a U-shaped molecule with a catalytic domain that superimposes closely on that of other pseudouridine synthases

Escherichia coli

Q57261

663378

sitting-drop vapour-diffusion method, crystals diffract to a minimum Bragg spacing of 2.4 A and belong to space group P212121, with unit-cell parameters a = 63.4, b = 108.6, c = 111.7 A

Escherichia coli

Q57261

649243