5.4.99.24: 23S rRNA pseudouridine955/2504/2580 synthase
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Reaction
Synonyms
pseudouridine synthases RluC, ribosomal large subunit pseudouridine synthase C, RLuc, YceC
ECTree
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Crystallization
Crystallization on EC 5.4.99.24 - 23S rRNA pseudouridine955/2504/2580 synthase
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a proteolytically derived fragment of the enzyme consisting of residues 89-319 retains catalytic activity. Crystals of this fragment, grown by precipitation with sodium acetate at pH 8.0, belong to space group P321, with unit-cell dimensions a = b = 97.1, c = 86.3 A and have two molecules in the crystallographic asymmetric unit. Crystals diffract X-rays to at least 2.3 A resolution and appear suitable for crystal structure determination
crystals of the catalytic domain RluC(92-319) are grown at 20°C using the hanging drop method