5.4.99.21: 23S rRNA pseudouridine2604 synthase
This is an abbreviated version!
For detailed information about 23S rRNA pseudouridine2604 synthase, go to the full flat file.
Reaction
Synonyms
RluF, YjbC
ECTree
5.4.99.21 23S rRNA pseudouridine2604 synthaseAdvanced search results
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Substrates Products on EC 5.4.99.21 - 23S rRNA pseudouridine2604 synthase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
23S rRNA uridine2604
23S rRNA pseudouridine2604
it appears that YjbC is not completely specific for uridine2604 but can, to a small extent also react with uridine2605. The enzyme has an essential aspartate residue, at position 107
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23S rRNA uridine2604
23S rRNA pseudouridine2604
RluF does not modify U2604 in rRNA mutant stem-loops that lack the A2602 bulge and shows dramatically higher activity for a stem-loop with a mutation designed to facilitate A2602 refolding into the stem with concomitant RNA strand translation. The substrate of RluF undergoes radical structural rearrangements upon binding. The RluF target base lies in an RNA stem rather than in a flexible loop and the substrate conformational change requires disruption of multiple base pairs. This energetically costly process is driven by interactions of the RNA with the protein
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