5.4.99.20: 23S rRNA pseudouridine2457 synthase
This is an abbreviated version!
For detailed information about 23S rRNA pseudouridine2457 synthase, go to the full flat file.
Reaction
Synonyms
pseudouridine synthase, RluE, YmfC
ECTree
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Substrates Products
Substrates Products on EC 5.4.99.20 - 23S rRNA pseudouridine2457 synthase
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REACTION DIAGRAM
23S rRNA uridine2457
23S rRNA pseudouridine2457
pseudouridine synthase RluE modifies U2457 in a stem of 23S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome.The stem alone is not a good RluE substrate, suggesting RluE undergoes additional interactions with other regions in the ribosome
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23S rRNA uridine2457
23S rRNA pseudouridine2457
pseudouridine synthase RluE modifies U2457 in a stem of 23S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome. The stem alone is not a good RluE substrate, suggesting RluE undergoes additional interactions with other regions in the ribosome
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23S rRNA uridine2457
23S rRNA pseudouridine2457
the enzyme has an essential Asp at postion 79
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23S rRNA uridine2457
23S rRNA pseudouridine2457
Escherichia coli pseudouridine synthase RluE targeting a single uridine in helix 89 (H89) in the peptidyltransferase center (PTC) of 23S rRNA. The target of RluE in H89 is Psi2457, analysis, overview
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23S rRNA uridine2457
23S rRNA pseudouridine2457
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Escherichia coli pseudouridine synthase RluE targeting a single uridine in helix 89 (H89) in the peptidyltransferase center (PTC) of 23S rRNA. The target of RluE in H89 is Psi2457, analysis, overview
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RluE acts as a stand-alone, highly specific enzyme forming the universally conserved pseudouridine at position 2457, located in helix 89 (H89) of the 23S rRNA in the peptidyltransferase center, detailed structure-function analysis to determine the structural elements both in RluE and in 23S rRNA required for RNA-protein interaction and pseudouridine formation
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additional information
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RluE recognizes a large part of 23S rRNA comprising both H89 and the single-stranded flanking regions which explains the high substrate specificity of RluE. Within RluE, the target RNA is recognized through sequence-specific contacts with loop L7-8 as well as interactions with loop L1-2 and the flexible N-terminal region
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additional information
?
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RluE acts as a stand-alone, highly specific enzyme forming the universally conserved pseudouridine at position 2457, located in helix 89 (H89) of the 23S rRNA in the peptidyltransferase center, detailed structure-function analysis to determine the structural elements both in RluE and in 23S rRNA required for RNA-protein interaction and pseudouridine formation
-
-
?
additional information
?
-
-
RluE recognizes a large part of 23S rRNA comprising both H89 and the single-stranded flanking regions which explains the high substrate specificity of RluE. Within RluE, the target RNA is recognized through sequence-specific contacts with loop L7-8 as well as interactions with loop L1-2 and the flexible N-terminal region
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-
?