5.4.99.20: 23S rRNA pseudouridine2457 synthase
This is an abbreviated version!
For detailed information about 23S rRNA pseudouridine2457 synthase, go to the full flat file.
Reaction
Synonyms
pseudouridine synthase, RluE, YmfC
ECTree
5.4.99.20 23S rRNA pseudouridine2457 synthaseAdvanced search results
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results (Engineering
Engineering on EC 5.4.99.20 - 23S rRNA pseudouridine2457 synthase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
R153A/P155G/R158A/R160A
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type
R196A
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type
R31A/R32A/K33A
site-directed mutagenesis, the mutant shows pseudouridylation activity similar to wild-type
R60A/K61A
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type
R153A/P155G/R158A/R160A
-
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type
-
R196A
-
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type
-
R31A/R32A/K33A
-
site-directed mutagenesis, the mutant shows pseudouridylation activity similar to wild-type
-
R60A/K61A
-
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type
-
additional information
additional information
generation of the deletion mutants RluE DELTAN(1-35) lacking the N-terminal region deleted at residue 35 and RluE DELTAR158-K161 lacking loop L7-8. Mutant RluE DELTAN(1-35) shows pseudouridylation activity similar to wild-type, while the activity of mutant RluE DELTAR158-K161 is significantly reduced
additional information
-
generation of the deletion mutants RluE DELTAN(1-35) lacking the N-terminal region deleted at residue 35 and RluE DELTAR158-K161 lacking loop L7-8. Mutant RluE DELTAN(1-35) shows pseudouridylation activity similar to wild-type, while the activity of mutant RluE DELTAR158-K161 is significantly reduced
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