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5.4.99.15: (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase

This is an abbreviated version!
For detailed information about (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase, go to the full flat file.

Word Map on EC 5.4.99.15

Reaction

4-[(1->4)-alpha-D-glucosyl]n-1-D-glucose
=
1-alpha-D-[(1->4)-alpha-D-glucosyl]n-1-alpha-D-glucopyranoside

Synonyms

(1,4)-alpha-D-glucan 1-alpha-D-glucosylmutase, (1->4)-alpha-D-Glucan 1-alpha-glucosylmutase, Cellulase (Arthrobacter strain Q36 clone pBMT13 trehalose oligosaccharide-forming), Cellulase (Rhizobium strain M11 clone pBMT7 trehalose oligosaccharide-forming), Malto-oligosyltrehalose synthase, Maltodextrin alpha-D-glucosyltransferase, maltooligosyl trehalose synthase, Maltooligosyl trehalose synthase (Rhizobium strain M-11 clone pBMTU1 gene treY reduced), maltooligosyl-trehalose synthase, maltooligosyltrehalose synthase, MOTS, MTS, MTSase, MTSH, protein ST0929, SEB24, SP3.5, SSO2095, SsTDFE, Synthase, maltooligosyltrehalose (Rhizobium strain M-11 clone pBMTU1 gene treY reduced), Synthase, maltooligosyltrehalose (Sulfolobus acidocaldarius clone pBST1 gene treY), Sythase, maltooligosyltrehalose (Arthrobacter clone pBRT4 gene treY), TDFE, trehalosyl dextrin-forming enzyme, TreY

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.15 (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase

Temperature Stability

Temperature Stability on EC 5.4.99.15 - (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
1 h, 65% loss of activity
40
-
60 min, stable up to
60 - 75
temperature stability of recombinant SEB24 and SP3.5, both of them have a half-life of 12 h at 75°C. The half-lives of SEB24 and SP3.5 at 60°C are 6 d and 6.5 d, respectively. SP3.5 and SEB24 retain 80% and 97% of their original activity after incubation for 2 days at 60°C
70
-
48 h, 80% residual activity
70 - 80
-
the MTSH fusion enzyme exhibits a high degree of thermostability, retaining 80% of the activity when pre-incubated at 70°C for 48 h, the stability is gradually abolished by incubating the fusion enzyme at above 80°C for 30 min
75
-
MTS proves stable at 75°C for more than 24 h, with a half life of about 50 h
75 - 85
purified recombinant enzyme, pH 5.0, 10 h, highly stable
90
-
1 h, 30% loss of activity