Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

5.4.99.15: (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase

This is an abbreviated version!
For detailed information about (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase, go to the full flat file.

Word Map on EC 5.4.99.15

Reaction

4-[(1->4)-alpha-D-glucosyl]n-1-D-glucose
=
1-alpha-D-[(1->4)-alpha-D-glucosyl]n-1-alpha-D-glucopyranoside

Synonyms

(1,4)-alpha-D-glucan 1-alpha-D-glucosylmutase, (1->4)-alpha-D-Glucan 1-alpha-glucosylmutase, Cellulase (Arthrobacter strain Q36 clone pBMT13 trehalose oligosaccharide-forming), Cellulase (Rhizobium strain M11 clone pBMT7 trehalose oligosaccharide-forming), Malto-oligosyltrehalose synthase, Maltodextrin alpha-D-glucosyltransferase, maltooligosyl trehalose synthase, Maltooligosyl trehalose synthase (Rhizobium strain M-11 clone pBMTU1 gene treY reduced), maltooligosyl-trehalose synthase, maltooligosyltrehalose synthase, MOTS, MTS, MTSase, MTSH, protein ST0929, SEB24, SP3.5, SSO2095, SsTDFE, Synthase, maltooligosyltrehalose (Rhizobium strain M-11 clone pBMTU1 gene treY reduced), Synthase, maltooligosyltrehalose (Sulfolobus acidocaldarius clone pBST1 gene treY), Sythase, maltooligosyltrehalose (Arthrobacter clone pBRT4 gene treY), TDFE, trehalosyl dextrin-forming enzyme, TreY

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.15 (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase

Cloned

Cloned on EC 5.4.99.15 - (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned from the genomic DNA of Sulfolobus solfataricus ATCC 35092 to an expression vector with a T7 lac promoter and then expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
EF433294
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli BL21(DE3)pLysS cells as fusion enzyme with maltooligosyltrehalose trehalohydrolase
-
expressed in Lactococcus lactis strain NZ9000
-
expression in Escherichia coli
gene mts, DNA and amino acid sequence determination and analysis, expression analysis
-
gene treY, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 CodonPlus(DE3)-RIL
inserted into an expression vector, pBV220, to yield the recombinant plasmid pSBGT1. MTSase gene in pBSGT1 is expressed in Escherichia coli
-
overexpression in Escherichia coli using the T7-expression system
-
overexpression of enzyme and maltooligosyltrehalose trehalohydrolase alone or with UPD-glucose pyrophosphorylase in a synthetic operon to improve trehalose production in Escherichia coli
-
the gene of maltooligosyltrehalose synthase is amplified by PCR. The primers are designed according to the published sequence of homologous gene from Sulfolobus acidocaldarius ATCC33909. This gene is inserted into the plasmid pBV220 and the resultant recombinant plasmid pBV220-GT is transformed to Escherichia coli DH5 alpha
-
two synthetic genes encoding the MTSase from Sulfolobus acidocaldarius strain ATCC 33909 are synthesized, recombinant expression of codon-optimized enzyme in Pichia pastoris strain KM71 (protein SP3.5) and in Escherichia coli strain BL21(DE3) (protein SEB24), method evaluation and optimization, overview
wild-type and mutant enzymes are expressed in Escherichia coli Rosetta (DE3)