5.4.99.12: tRNA pseudouridine38-40 synthase

This is an abbreviated version!
For detailed information about tRNA pseudouridine38-40 synthase, go to the full flat file.

Reaction

Synonyms

Deg1, Deg1p, hisT, mPus3p, pseudouridine synthase, pseudouridine synthase 3, pseudouridine synthase I, PSUI, PUS3, Pus3p, tRNA pseudouridine synthase 3, tRNA pseudouridine synthase I, TruA

ECTree

     5 Isomerases

         5.4 Intramolecular transferases

             5.4.99 Transferring other groups

                5.4.99.12 tRNA pseudouridine38-40 synthase

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Results	in table
1	Activating Compound
48337	AA Sequence
2	CAS Registry Number
11	Cloned(Commentary)
1	Cofactor
6	Crystallization (Commentary)
1	Expression
9	General Information
13	Inhibitors
8	kcat/KM [mM/s]
1	Ki Value [mM]
10	KM Value [mM]
2	Localization
1	Metals/Ions
4	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
109	Organism
17	PDB ID
1	pH Optimum
27	Protein Variants
4	Purification (Commentary)
4	Reaction
20	Reference
1	Source Tissue
1	Storage Stability
36	Substrates and Products (Substrate)
8	Subunits
23	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Stability [°C]
6	Turnover Number [1/s]

Reaction

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Reaction on EC 5.4.99.12 - tRNA pseudouridine38-40 synthase

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

tRNA uridine38-40 = tRNA pseudouridine38-40

4 entries

tRNA uridine38-40 = tRNA pseudouridine38-40

chemical evidence against a covalent cysteine intermediate in the rearrangement mechanism of uridine to pseudouridine

Escherichia coli

-

704385

tRNA uridine38-40 = tRNA pseudouridine38-40

structural, computational, and functional studies provide the basis for a substrate recognition model for the regional selectivity. By binding to the conserved parts of tRNAs (elbow and D stem backbone), TruA recognizes multiple tRNAs independent of sequence variations. Anchored at these two regions, TruA positions the anticodon stem loop near the active site without constraining its flexibility, thereby increasing the effective concentration of each target position, 38, 39, and 40, in the vicinity of the active site. The thermal motions of the anticodon stem loop allow the nucleotides at each of the three sites to be dynamically accessible for modification. TruA utilizes the intrinsic flexibility of the anticodon stem loop for site promiscuity and also to select against intrinsically stable tRNAs

Escherichia coli

P07649

681882

tRNA uridine38-40 = tRNA pseudouridine38-40

the mechanism of pseudouridine synthase I is deduced from its interaction with 5-fluorouracil-tRNA. The covalent complex formed between pseudouridine synthase I and 5-fluorouracil-tRNA involves Michael adduct formation between Asp60 of pseudouridine synthase I and the 6-carbon of 5-fluorouracil39 of tRNA to form a covalent pseudouridine synthase I-5-fluorouracil-tRNA complex

Escherichia coli

P07649

653624

tRNA uridine38-40 = tRNA pseudouridine38-40

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