5.4.99.12: tRNA pseudouridine38-40 synthase

This is an abbreviated version!
For detailed information about tRNA pseudouridine38-40 synthase, go to the full flat file.

Reaction

Synonyms

Deg1, Deg1p, hisT, mPus3p, pseudouridine synthase, pseudouridine synthase 3, pseudouridine synthase I, PSUI, PUS3, Pus3p, tRNA pseudouridine synthase 3, tRNA pseudouridine synthase I, TruA

ECTree

     5 Isomerases

         5.4 Intramolecular transferases

             5.4.99 Transferring other groups

                5.4.99.12 tRNA pseudouridine38-40 synthase

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Results	in table
1	Activating Compound
48337	AA Sequence
2	CAS Registry Number
11	Cloned(Commentary)
1	Cofactor
6	Crystallization (Commentary)
1	Expression
9	General Information
13	Inhibitors
8	kcat/KM [mM/s]
1	Ki Value [mM]
10	KM Value [mM]
2	Localization
1	Metals/Ions
4	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
109	Organism
17	PDB ID
1	pH Optimum
27	Protein Variants
4	Purification (Commentary)
4	Reaction
20	Reference
1	Source Tissue
1	Storage Stability
36	Substrates and Products (Substrate)
8	Subunits
23	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Stability [°C]
6	Turnover Number [1/s]

Inhibitors

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Inhibitors on EC 5.4.99.12 - tRNA pseudouridine38-40 synthase

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

5-fluorouracil tRNAPhe

2 entries

-

Calf thymus DNA

Bos taurus

-

native and denatured, about 66% residual activity

648848

-

Dithionitrobenzoate

Escherichia coli

-

irreversibly inactivates

648849

fluorouracil-substituted tRNA

Escherichia coli

P07649

causes a time-dependent inactivation of pseudouridine synthase I and forms a covalent complex with the enzyme that involves the fluorouracil-substituted UMP at position 39. Upon incubation of 100 nM pseudouridine synthase with 0.001 mM fluorouracil-substituted tRNA at 15°C prior to addition of substrate, there is a time-dependent inactivation of the enzyme with a half-life of 35 min

702197

-

iodoacetate

Escherichia coli

-

irreversibly inactivates

648849

p-chloromercuribenzoate

Escherichia coli

-

irreversibly inactivates

648849

poly(rA)n

Bos taurus

-

71% residual acivity

648848

-

poly(rC)n

Bos taurus

-

93% residual acivity

648848

-

poly(rG)n

Bos taurus

-

3.2% residual acivity

648848

-

poly(rI)n

Bos taurus

-

11.9% residual acivity

648848

-

poly(rU)n

Bos taurus

-

44% residual acivity

648848

-

tRNA

Bos taurus

-

activity is inhibited by single tRNAs or bulk tRNA from all sources tested, and by ribosomal RNA

648848

5-fluorouracil tRNAPhe

Escherichia coli

P07649

time-dependent inactivation of pseudouridine synthase I and formation of a covalent complex with the enzyme that involves the 5-fluorouracil monophosphate at position 39

702197

-

5-fluorouracil tRNAPhe

Saccharomyces cerevisiae

-

enzyme forms a covalent adduct with 5-fluorouracil-tRNA to form a putative analog of a steady-state intermediate in the normal reaction pathway. The putative Asp nucleophile is attached to the 6-position of the target 5-fluorouracil-tRNA to form a stable covalent adduct, which can undergo O-acyl hydrolytic cleavage, and the intermediate contains an intact N-glycosidic bond linking the modified base to the polynucleotide chain

653624

-