Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

5.4.99.11: isomaltulose synthase

This is an abbreviated version!
For detailed information about isomaltulose synthase, go to the full flat file.

Word Map on EC 5.4.99.11

Reaction

sucrose
=
6-O-alpha-D-glucopyranosyl-D-fructofuranose

Synonyms

alpha-Glucosyltransferase, alpha-glycosyl transferase, Avin_08330, AZOG, EC 5.4.99.10, esi, isomaltulose synthase, Isomaltulose synthetase, MutB, NX-5, PalI, Pall, PdSIase, SIase, SmuA, Sucrose 6-glucosylmutase, Sucrose alpha-glucosyltransferase, sucrose isomerase, Sucrose mutase, Synthase, isomaltulose, Trehalulose synthase

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.11 isomaltulose synthase

Temperature Stability

Temperature Stability on EC 5.4.99.11 - isomaltulose synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 40
recombinant enzyme, completely stable at
20 - 70
about 50% activity at 20°C, about 80% activity at 30°C, 100% activity at 40-50°C, about 20% activity at 55°C, complete loss of activity at 60-70°C
30 - 60
the enzyme is stable for longer than 48 h at 30°C. The stability of the enzyme decreases drastically at 50°C and 60°C with a half-life duration of 90 min and 5 min, respectively
35 - 55
-
recombinant enzyme displayed on Saccharomyces cerevisiae surface, stable at, half-life at 50°C is 2.4 h
40
Paraburkholderia acidicola
-
1 h, pH 5.6, stable up to
45
at 45°C and pH 6.0, the half-life of wild-type enzyme is 39.2 minutes. Whereas, the half-lives of mutants E175N, K576D, and E175N/K576D are 90.2 minutes, 69.8 minutes, and 300 minutes, respectively, which are 2.30, 1.78 and 7.65 times greater than that of the wild-type enzyme
52
50% activity remaining of recombinant enzyme expressed from Escherichia coli, inactivation at 65°C, profile overview