5.4.99.11: isomaltulose synthase
This is an abbreviated version!
For detailed information about isomaltulose synthase, go to the full flat file.
Word Map on EC 5.4.99.11
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5.4.99.11
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synthesis
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erwinia
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rhapontici
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palatinose
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protaminobacter
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2.4.1.1
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pantoea
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1,4-alpha-d-glucan:orthophosphate
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molasses
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plymuthica
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food industry
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vacuole-targeted
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dispersa
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whiteflies
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bemisia
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honeydew
- 5.4.99.11
- synthesis
- erwinia
- rhapontici
- palatinose
-
protaminobacter
-
2.4.1.1
- pantoea
-
1,4-alpha-d-glucan:orthophosphate
- molasses
- plymuthica
- food industry
-
vacuole-targeted
- dispersa
- whiteflies
- bemisia
- honeydew
Reaction
Synonyms
alpha-Glucosyltransferase, alpha-glycosyl transferase, Avin_08330, AZOG, EC 5.4.99.10, esi, isomaltulose synthase, Isomaltulose synthetase, MutB, NX-5, PalI, Pall, PdSIase, SIase, SmuA, Sucrose 6-glucosylmutase, Sucrose alpha-glucosyltransferase, sucrose isomerase, Sucrose mutase, Synthase, isomaltulose, Trehalulose synthase
ECTree
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Temperature Stability
Temperature Stability on EC 5.4.99.11 - isomaltulose synthase
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20 - 70
about 50% activity at 20°C, about 80% activity at 30°C, 100% activity at 40-50°C, about 20% activity at 55°C, complete loss of activity at 60-70°C
22
30
30 - 60
the enzyme is stable for longer than 48 h at 30°C. The stability of the enzyme decreases drastically at 50°C and 60°C with a half-life duration of 90 min and 5 min, respectively
35 - 55
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recombinant enzyme displayed on Saccharomyces cerevisiae surface, stable at, half-life at 50°C is 2.4 h
45
at 45°C and pH 6.0, the half-life of wild-type enzyme is 39.2 minutes. Whereas, the half-lives of mutants E175N, K576D, and E175N/K576D are 90.2 minutes, 69.8 minutes, and 300 minutes, respectively, which are 2.30, 1.78 and 7.65 times greater than that of the wild-type enzyme
50
52
50% activity remaining of recombinant enzyme expressed from Escherichia coli, inactivation at 65°C, profile overview
60
50
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half-life of wild-type enzyme: 1.81 min, half-life of mutant enzymes E498P is 9.45 min, half-life of mutant enzyme E498P/R310P is 13.61 min
60
about 50% activity remaining of recombinant enzyme expressed from Yarrowia lipolytica, inactivation at 70°C, profile overview