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5.4.99.11: isomaltulose synthase

This is an abbreviated version!
For detailed information about isomaltulose synthase, go to the full flat file.

Word Map on EC 5.4.99.11

Reaction

sucrose
=
6-O-alpha-D-glucopyranosyl-D-fructofuranose

Synonyms

alpha-Glucosyltransferase, alpha-glycosyl transferase, Avin_08330, AZOG, EC 5.4.99.10, esi, isomaltulose synthase, Isomaltulose synthetase, MutB, NX-5, PalI, Pall, PdSIase, SIase, SmuA, Sucrose 6-glucosylmutase, Sucrose alpha-glucosyltransferase, sucrose isomerase, Sucrose mutase, Synthase, isomaltulose, Trehalulose synthase

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.11 isomaltulose synthase

Specific Activity

Specific Activity on EC 5.4.99.11 - isomaltulose synthase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0072
pH 7.0, 30°C, purified recombinant mutant F297A+P
0.046
pH 7.0, 30°C, purified recombinant mutant F297A
0.11
recombinant enzyme, pH 6.0, 35°C, substrate sucrose
0.14
pH 7.0, 30°C, purified recombinant mutant R333K
0.52
recombinant enzyme, pH 6.0, 35°C, substrate kojibiose
0.6
recombinant enzyme, pH 6.0, 35°C, substrate isomaltotriose
0.97
recombinant enzyme, pH 6.0, 35°C, substrate methyl alpha-D-glucoside
0.98
recombinant enzyme, pH 6.0, 35°C, substrate isomaltose
1.1
pH 7.0, 30°C, purified recombinant wild-type enzyme
1017.6
purified recombinant mutant E175N pH 6.0, 30°C, substrate sucrose
1045.7
purified recombinant mutant K576D, pH 6.0, 30°C, substrate sucrose
120
-
native enzyme, pH not specified in the publication, 30°C
1218.9
purified recombinant mutant E175N/K576D, pH 6.0, 30°C, substrate sucrose
13.98
Paraburkholderia acidicola
substrate sucrose, pH not specified in the publication, 20°C, native enzyme
181
Bemisia argentifolia
-
sucrose
19.8
-
pH not specified in the publication, 40°C, native enzyme
2
-
crude extract, in 25 mM PBS (pH 5.8), at 30°C
2.16
-
crude enzyme, pH 6.3, 33°C
2.33
recombinant enzyme, pH 6.0, 35°C, substrate isomaltulose
2.9
recombinant enzyme, pH 6.0, 35°C, substrate 4-nitrophenyl alpha-D-trehalose
280
-
sucrose
328
recombinant enzyme, pH not specified in the publication, 35°C
38.75
-
purified native enzyme, pH 6.3, 33°C
423
pH not specified in the publication, 30°C, native enzyme
427.1
-
after 213.6fold purification, in 25 mM PBS (pH 5.8), at 30°C
500
-
pH 5.5, 25°C
6
-
free recombinant enzyme, pH 6.0, 30°C
65.3
-
sucrose
71.58
-
immobilized recombinant enzyme, pH 6.0, 30°C
957.5
purified recombinant wild-type enzyme, pH 6.0, 30°C, substrate sucrose
additional information