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5.4.99.11: isomaltulose synthase

This is an abbreviated version!
For detailed information about isomaltulose synthase, go to the full flat file.

Word Map on EC 5.4.99.11

Reaction

sucrose
=
6-O-alpha-D-glucopyranosyl-D-fructofuranose

Synonyms

alpha-Glucosyltransferase, alpha-glycosyl transferase, Avin_08330, AZOG, EC 5.4.99.10, esi, isomaltulose synthase, Isomaltulose synthetase, MutB, NX-5, PalI, Pall, PdSIase, SIase, SmuA, Sucrose 6-glucosylmutase, Sucrose alpha-glucosyltransferase, sucrose isomerase, Sucrose mutase, Synthase, isomaltulose, Trehalulose synthase

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.11 isomaltulose synthase

Crystallization

Crystallization on EC 5.4.99.11 - isomaltulose synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
wild-type NX-5 and enzyme mutant complexes E295Q-sucrose and D241A-glucose, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein solution with 0.001 ml of reservoir solution containing 0.1 M trisodium citrate, pH 5.6, 0.2 M ammonium acetate, and 30% PEG 4000, and for sucrose-complexed mutant enzyme crystal 20 mM sucrose, 0.1 M bicine, pH 9.0, and 30% PEG 6000. The D241A-glucose complex and R335H/R336T/K337I/D338P-glucose complex are co-crystallized in the presence of 20 mM sucrose, 0.1 M sodium cacodylate, pH 6.5, and 29% PEG 8000, 20°C, 3-5 days, X-ray diffraction structure determination and analysis at 1.70 A, 1.70 a, and 2.00 A resolutions, respectively
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hanging-drop vapour-diffusion method at 295 K
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crystal structure determination, PDB ID 1M53
hanging-drop vapor diffusion method, structure solved at 2.2 A resolution
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crystal structure determinations of native enzyme and enzyme mutants D200A and E254Q, PDB IDs 1ZJB, 2PWH, 1ZJA, 2PWD, 2PWG, 2PWF, and 2PWE, interacting compounds are Ca2+, sucrose, D-glucose, tromethamine, Tris, 1-deoxynojirimycin, and castanospermine
Paraburkholderia acidicola
expression in Escherichia coli
Paraburkholderia acidicola
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wild-type in native state and in complex with competitive inhibitors Tris, deoxynojirimycin, and castanospermine, and inactive mutants D200A, and E254Q, in complex with D-glucose and sucrose
Paraburkholderia acidicola
-
purified recombinant enzyme mutants R284C in complex with transition-state mimic deoxynojirimycin, and F164L in complex with glucose, sucrose, trehalulose, or isomaltulose, X-ray diffraction structure determination and analysis at 1.7-2.15 A resolution
crystal structure determinations of enzyme mutants F164L and R284C, PDB IDs 4H2C, 4GIN, 4GI6, 4GI8, 4GIA, and 4GI9, interacting compounds are Ca2+, glycerin, D-glucose, and Tris
crystal structure determination, PDB IDs GBD and GBE, with ethylene glycol, citrate, 1,2-ethanediol, and 1-deoxynojirimycin as interacting compounds
-
in complex with deoxynojirimycin, sitting drop vapor diffusion method, using
native enzyme, crystals belong to space group P212121 and diffract to 1.95 A resolution
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