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5.4.4.2: isochorismate synthase

This is an abbreviated version!
For detailed information about isochorismate synthase, go to the full flat file.

Word Map on EC 5.4.4.2

Reaction

chorismate
=
isochorismate

Synonyms

Amonabactin, AtICS1, AtICS2, DhbC, EC 5.4.99.6, Eds16, EntC, ICS, ICS1, ICS1 gene product, ICS2, IcsI, isochorismatase, isochorismate hydroxymutase, isochorismate mutase, isochorismate mutase 1, isochorismate mutase 2, isochorismate synthase, isochorismate synthase 1, isochorismate synthase 1, chloroplastic, isochorismate synthase 2, chloroplastic, Isochorismic synthase, MbtI, menaquinone-specific isochorismate synthase, MenF, PchA, salicylate biosynthesis isochorismate synthase, salicylate synthase, Sid2, Synthase, isochorismate

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.4 Transferring hydroxy groups
                5.4.4.2 isochorismate synthase

Engineering

Engineering on EC 5.4.4.2 - isochorismate synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A303T
the kcat/Km (chorismate) for the A303T mutant is 130fold lower than that for wild type enzyme
A303T/F327Y
the mutant shows no detectable activity
A303T/F327Y/F359Q
the mutant shows no detectable activity
A303T/F327Y/F359Q/I346L
the mutant shows no detectable activity
A303T/F327Y/I346L
the mutant shows no detectable activity
A303T/F359Q
the mutant shows no detectable activity
A344T
no catalytic activity
E240Q
no catalytic activity
F327Y
the mutant displays reduced activity compared to the wild type enzyme
F327Y/F359Q
the mutant displays a reduced kcat/Km value compared to the wild type enzyme
F327Y/F359Q/I346L
the mutant shows no detectable activity
F359Q
F359Q/I346L
the mutant displays a reduced kcat/Km value compared to the wild type enzyme
I346L
the EntC mutant has a 12fold lower kcat/Km (chorismate) than the wild type enzyme
K190A
inactive mutant
K90A
no catalytic activity
L255A
50% increase in Km value, 90% decrease in kcat value
L304A
the mutation causes loss of catalytic activity
L304A/F359Q
the mutant shows no detectable activity
R387A
no catalytic activity
E269A
-
site-directed mutagenesis, the residue acts as general acid, inactive mutant
E313A
-
site-directed mutagenesis, the residue chelates Mg2+ and acts as general base, mutant with highly reduced activity compared to wild-type
E313Q
-
site-directed mutagenesis, the residue chelates Mg2+ and acts as general base, mutant with highly reduced activity compared to wild-type
K221A
-
site-directed mutagenesis, the residue acts as general base, mutant with highly reduced activity compared to wild-type
additional information