5.4.4.2: isochorismate synthase
This is an abbreviated version!
For detailed information about isochorismate synthase, go to the full flat file.
Word Map on EC 5.4.4.2
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5.4.4.2
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corticosteroid
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inhaled
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asthma
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children
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pulmonary
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long-acting
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airway
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labas
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asthmatic
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agonist
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bronchodilator
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placebo
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fluticasone
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eosinophil
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expiratory
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prescript
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short-acting
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double-blind
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physician
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bronchial
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beta2-agonists
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budesonide
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spirometry
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montelukast
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add-on
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wheezing
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randomised
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ketanserin
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5-hydroxytryptamine
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leukotriene
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once-daily
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muscarinic
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monotherapy
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salmeterol
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sputum
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5-ht-induced
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urodynamic
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parallel-group
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methysergide
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intercostal
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fixed-dose
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immunochromatographic
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self-management
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ondansetron
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incontinence
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real-life
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drug development
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8-oh-dpat
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datalink
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agriculture
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airflow
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run-in
- 5.4.4.2
-
corticosteroid
-
inhaled
- asthma
- children
- pulmonary
-
long-acting
- airway
-
labas
-
asthmatic
- agonist
-
bronchodilator
-
placebo
-
fluticasone
-
eosinophil
-
expiratory
-
prescript
-
short-acting
-
double-blind
-
physician
- bronchial
-
beta2-agonists
-
budesonide
-
spirometry
- montelukast
-
add-on
-
wheezing
-
randomised
- ketanserin
- 5-hydroxytryptamine
-
leukotriene
-
once-daily
-
muscarinic
-
monotherapy
- salmeterol
- sputum
-
5-ht-induced
-
urodynamic
-
parallel-group
- methysergide
-
intercostal
-
fixed-dose
-
immunochromatographic
-
self-management
- ondansetron
- incontinence
-
real-life
- drug development
-
8-oh-dpat
-
datalink
- agriculture
-
airflow
-
run-in
Reaction
Synonyms
Amonabactin, AtICS1, AtICS2, DhbC, EC 5.4.99.6, Eds16, EntC, ICS, ICS1, ICS1 gene product, ICS2, IcsI, isochorismatase, isochorismate hydroxymutase, isochorismate mutase, isochorismate mutase 1, isochorismate mutase 2, isochorismate synthase, isochorismate synthase 1, isochorismate synthase 1, chloroplastic, isochorismate synthase 2, chloroplastic, Isochorismic synthase, MbtI, menaquinone-specific isochorismate synthase, MenF, PchA, salicylate biosynthesis isochorismate synthase, salicylate synthase, Sid2, Synthase, isochorismate
ECTree
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Engineering
Engineering on EC 5.4.4.2 - isochorismate synthase
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A303T
the kcat/Km (chorismate) for the A303T mutant is 130fold lower than that for wild type enzyme
F327Y
the mutant displays reduced activity compared to the wild type enzyme
F327Y/F359Q
the mutant displays a reduced kcat/Km value compared to the wild type enzyme
F359Q
F359Q/I346L
the mutant displays a reduced kcat/Km value compared to the wild type enzyme
I346L
the EntC mutant has a 12fold lower kcat/Km (chorismate) than the wild type enzyme
E269A
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site-directed mutagenesis, the residue acts as general acid, inactive mutant
E313A
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site-directed mutagenesis, the residue chelates Mg2+ and acts as general base, mutant with highly reduced activity compared to wild-type
E313Q
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site-directed mutagenesis, the residue chelates Mg2+ and acts as general base, mutant with highly reduced activity compared to wild-type
K221A
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site-directed mutagenesis, the residue acts as general base, mutant with highly reduced activity compared to wild-type
additional information
the mutant displays a reduced kcat/Km value compared to the wild type enzyme
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plants defective in enzyme activity due to mutation sid2, level of salicylic acid and the activity of enzyme do not increase in response to O3 exposure
additional information
genetic mapping and genome sequencing indicate that mutation sed111 is in the gene salicylic acid induction-deficient 2 (SID2), which encodes isochorismate synthase 1, ICS1. Mutation sed111 belongs to a series of mutants called suppressor of esd4 (sed), which delay flowering, enhance growth and reduce hyperaccumulation of SUMO conjugates. Mutations in the SUMO protease early in short days 4 (ESD4) cause hyperaccumulation of conjugates formed between SUMO and its substrates, and phenotypically are associated with extreme early flowering and impaired growth. Compared to wild-type plants, esd4 contains higher levels of SID2 mRNA and about threefold more salicylate, whereas sed111 contains lower salicylate levels. Other sed mutations also reduce the high SID2 mRNA abundance and free salicylate levels found in esd4-1. Sed, esd, and sid mutation analysis in Arabidopsis thaliana, detailed overview