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5.4.4.2: isochorismate synthase

This is an abbreviated version!
For detailed information about isochorismate synthase, go to the full flat file.

Word Map on EC 5.4.4.2

Reaction

chorismate
=
isochorismate

Synonyms

Amonabactin, AtICS1, AtICS2, DhbC, EC 5.4.99.6, Eds16, EntC, ICS, ICS1, ICS1 gene product, ICS2, IcsI, isochorismatase, isochorismate hydroxymutase, isochorismate mutase, isochorismate mutase 1, isochorismate mutase 2, isochorismate synthase, isochorismate synthase 1, isochorismate synthase 1, chloroplastic, isochorismate synthase 2, chloroplastic, Isochorismic synthase, MbtI, menaquinone-specific isochorismate synthase, MenF, PchA, salicylate biosynthesis isochorismate synthase, salicylate synthase, Sid2, Synthase, isochorismate

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.4 Transferring hydroxy groups
                5.4.4.2 isochorismate synthase

Cloned

Cloned on EC 5.4.4.2 - isochorismate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
expressed in Arabidopsis thaliana sid2-2 mutant
-
expressed in Brassica rapa subsp. oleifera
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli strain BL21 (DE3)
expression in Escherichia coli
gene dhbC, overexpression in Escherichia coli strain BL21-CodonPlus(DE3)-RIPL as His-tagged enzyme from vector pMCSG19c in fusion N-terminally with maltose binding protein and a Tobacco vein mottling virus protease cleaving site, the His-tag is cleavable by Tobacco etch virus protease, in M9 SeMET High-Yield growth medium
Q81QQ0
gene ICS, DNA and amino acid sequence determination and analysis, sequence comparisons, real-time PCR enzyme expression analysis
-
gene ICS1, located on chromosome 1, cloning from seedling cDNA library, recombinant expression of His6-tagged isozyme ICS1 in Escherichia coli strain Rosetta DE3 pLysS
gene ICS1, yeast one-hybrid (Y1H) screening and transactivation activity assay in Saccharomyces cerevisiae strain YM4271
gene ICS2, located on chromosome 1, cloning from pFL61 library, functional recombinant expression of His6-tagged isozyme ICS2 in Escherichia coli strain Rosetta DE3 pLysS
gene pchA, overexpression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS
-
overexpression in Escherichia coli BL21(DE3)
-
sequence comparisons