5.4.3.6: tyrosine 2,3-aminomutase

This is an abbreviated version!
For detailed information about tyrosine 2,3-aminomutase, go to the full flat file.

Word Map on EC 5.4.3.6

5.4.3.6

enediyne

ammonia

chromophore

crocatus

sativa

lyases

chondromyces

stereochemistry

myxobacterium

enantioselectivity

isomerizes

globisporus

peptidyl

prosthetic

chromoprotein

taxus

enantiomeric

fluorinated

protein-tethered

fad-dependent

depsipeptides

acrylate

pathway-specific

toney

lyase-like

Reaction

Synonyms

3,5-dihydro-5-methylidene-4H-imidazol-4-one-dependent aminomutase, 3,5-dihydro-5-methylidine-4H-imidazol-4-one-dependent tyrosine aminomutase, Aminomutase, tyrosine 2,3-, CmdF, MfTAM, MIO-dependent aminomutase, MIO-dependent tyrosine aminomutase, More, MxTAM, OsTAM, SgcC4, TAM, Tam1, Tyrosine alpha,beta-amino mutase, Tyrosine alpha,beta-mutase, tyrosine aminomutase

ECTree

5 Isomerases

5.4 Intramolecular transferases

5.4.3 Transferring amino groups

5.4.3.6 tyrosine 2,3-aminomutase

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Results	in table
152	AA Sequence
1	CAS Registry Number
10	Cloned(Commentary)
4	Cofactor
2	Crystallization (Commentary)
2	Expression
9	General Information
1	General Stability
6	IC50 Value
18	Inhibitors
10	kcat/KM [mM/s]
16	KM Value [mM]
2	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
16	Organism
4	Pathway
8	PDB ID
7	pH Optimum
2	pH Range
43	Protein Variants
8	Purification (Commentary)
5	Reaction
5	Reaction Type
16	Reference
5	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
35	Substrates and Products (Substrate)
2	Subunits
35	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
18	Turnover Number [1/s]

Purification

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Purification on EC 5.4.3.6 - tyrosine 2,3-aminomutase

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Brevibacillus brevis

3405

Ni-NTA resin column chromatography, HiTrap Q column chromatography, and Superdex 200 gel filtration

Streptomyces globisporus

714481

purification is carried out by affinity chromatography with GST-Sepharose, the GST-tag is cleaved by using the PreScission protease

3 entries

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration

Chondromyces crocatus

727685

recombinant His-tagged wild-type and mutant enzymes by nickel affinity chromatography

Oryza sativa

747104

recombinant tyrosine aminomutase

Streptomyces globisporus

650190