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5.4.2.2: phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)

This is an abbreviated version!
For detailed information about phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent), go to the full flat file.

Word Map on EC 5.4.2.2

Reaction

alpha-D-glucose 1-phosphate
=
D-glucose 6-phosphate

Synonyms

alpha phosphoglucomutase, alpha-Pgm, alpha-phosphoglucomutase, alpha-phosphoglucomutase 1, alpha-phosphoglucose mutase, cPGM, cytosolic phosphoglucomutase, EC 2.7.5.1, Glucose phosphomutase, PGM, PGM-I, PGM-II, PGM/PMM, PGM1, PGM2, PGM3, PgmA, PgmG, phospho-glucomutase, phosphoglucomutase, phosphoglucomutase 1, phosphoglucomutase/phosphomannomutase, phosphoglucomutase1, Phosphoglucose mutase, Phosphohexomutase, phosphomannomutase/phosphoglucomutase, Phosphomutase, glucose, plastidic phosphoglucomutase, PMM/PGM, pPGM, SP-1, SpgM, SSO0207, XanA, YMR278w protein

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.2 Phosphotransferases (phosphomutases)
                5.4.2.2 phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)

Purification

Purification on EC 5.4.2.2 - phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isozymes
-
ammonium sulfate, Sephadex G-25, DEAE-Toyopearl, Toyopearl HW-65
-
both native and recombinant enzyme
glutathione Sepharose column chromatography
heat treatment 85°C, 20 min, anion-exchange, hydrophobic and gel filtration column chromatography
-
His-Select Ni-Affinity gel column chromatography, gel filtration
isozymes PGM1 and PGM2
-
maltose binding protein-Trap column chromatography and Superdex 200 gel filtration
-
Ni-chelating resin chromatography
Ni-NTA agarose column chromatography
Ni-NTA column chromatography
Ni-Sepharose affinity column chromatography
-
Ni2+-NTA column chromatography
-
nickel-affinity column chromatography
-
partial
Q-Sepharose column chromatography and Sephacryl-S 100 gel filtration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3), removal of the His-tag, purification by nickel affinity chromatography and dialysis
-
recombinant His-tagged Pgm1, Pgm2, and Pgm3 from Escherichia coli strain BL21 (DE3)
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, protein refolding, and ultrafiltration
recombinant PGM
recombinant protein is purified by centrifugation und affinity chromatography with Ni-NTA Superflow cartridge. Purity is assessed by SDS-PAGE
recombinant untagged mutant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography and dialysis, recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
ultracentrifugation
-