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5.4.2.10: phosphoglucosamine mutase

This is an abbreviated version!
For detailed information about phosphoglucosamine mutase, go to the full flat file.

Word Map on EC 5.4.2.10

Reaction

alpha-D-glucosamine 1-phosphate
=
D-glucosamine 6-phosphate

Synonyms

alpha-D-glucosamine 1,6-phosphomutase, alpha-phosphoglucoseamine mutase, alpha-PNGM, aminodeoxyglucose phosphate phosphomutase, Aq1627, GlmM, glucosamine phosphomutase, MMP1077, phosphoglucosamine mutase, PNGM

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.2 Phosphotransferases (phosphomutases)
                5.4.2.10 phosphoglucosamine mutase

Cloned

Cloned on EC 5.4.2.10 - phosphoglucosamine mutase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 Star (DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
gene glmM, DNA and amino acid sequence determination and analysis, transcriptional and expression analysis, recombinant overexpression in Lactobacillus casei BL23 leading to a 6.43fold increased enzyme activity
gene glmM, subcloning in Escherichia coli strain NOvaBlue
-
into the pDONR221 vector and subsequently into the pDEST17 vector for expression in Escherichia coli BL21DE3 cells
-
into the pFW5 (Spec_r) Escherichia coli - streptococcus shuttle plasmid to create the mutant glmM gene, into the shuttle vector pDL289 to complement the glmM-deficient mutant strain
overexpression in Escherichia coli