5.4.2.10: phosphoglucosamine mutase
This is an abbreviated version!
For detailed information about phosphoglucosamine mutase, go to the full flat file.
Word Map on EC 5.4.2.10
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5.4.2.10
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glmm
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peptidoglycan
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glucosamine-1-phosphate
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udp-n-acetylglucosamine
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phosphoglucomutase
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udp-glcnac
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5'-diphospho-n-acetylglucosamine
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diadenylate
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uridyltransferase
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penicillin-binding
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mutases
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gordonii
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c-di-amp
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mengin-lecreulx
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methicillin
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medicine
- 5.4.2.10
- glmm
- peptidoglycan
-
glucosamine-1-phosphate
- udp-n-acetylglucosamine
- phosphoglucomutase
- udp-glcnac
-
5'-diphospho-n-acetylglucosamine
-
diadenylate
- uridyltransferase
-
penicillin-binding
- mutases
- gordonii
-
c-di-amp
-
mengin-lecreulx
- methicillin
- medicine
Reaction
Synonyms
alpha-D-glucosamine 1,6-phosphomutase, alpha-phosphoglucoseamine mutase, alpha-PNGM, aminodeoxyglucose phosphate phosphomutase, Aq1627, GlmM, glucosamine phosphomutase, MMP1077, phosphoglucosamine mutase, PNGM
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Cloned
Cloned on EC 5.4.2.10 - phosphoglucosamine mutase
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gene glmM, DNA and amino acid sequence determination and analysis, transcriptional and expression analysis, recombinant overexpression in Lactobacillus casei BL23 leading to a 6.43fold increased enzyme activity
into the pDONR221 vector and subsequently into the pDEST17 vector for expression in Escherichia coli BL21DE3 cells
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into the pFW5 (Spec_r) Escherichia coli - streptococcus shuttle plasmid to create the mutant glmM gene, into the shuttle vector pDL289 to complement the glmM-deficient mutant strain
overexpression in Escherichia coli