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5.3.99.6: allene-oxide cyclase

This is an abbreviated version!
For detailed information about allene-oxide cyclase, go to the full flat file.

Word Map on EC 5.3.99.6

Reaction

(9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate
=
(15Z)-12-oxophyto-10,15-dienoate

Synonyms

AaAOC, Allene oxide cyclase, allene oxide oxidase, allene oxide oxidase 3, AOC, AOC1, AOC2, AOC3, Cyclase, allene oxide, EC 5.3.99.1, JcAOC, OsAOC, PnAOC

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.99 Other intramolecular oxidoreductases
                5.3.99.6 allene-oxide cyclase

Purification

Purification on EC 5.3.99.6 - allene-oxide cyclase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography
-
Ni-NTA column chromatography
on a Ni-NTA column
recombinant GST-tagged OsAOC lacking the chloroplast transit peptide from Escherichia coli strain BL21 by glutathione affinity chromatography
Q8L6H4
recombinant N-terminally GST-tagged isozyme AOC1 from Escherichia coli by glutathione affinity chromatography and gel filtration