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5.3.99.6: allene-oxide cyclase

This is an abbreviated version!
For detailed information about allene-oxide cyclase, go to the full flat file.

Word Map on EC 5.3.99.6

Reaction

(9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate
=
(15Z)-12-oxophyto-10,15-dienoate

Synonyms

AaAOC, Allene oxide cyclase, allene oxide oxidase, allene oxide oxidase 3, AOC, AOC1, AOC2, AOC3, Cyclase, allene oxide, EC 5.3.99.1, JcAOC, OsAOC, PnAOC

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.99 Other intramolecular oxidoreductases
                5.3.99.6 allene-oxide cyclase

Cloned

Cloned on EC 5.3.99.6 - allene-oxide cyclase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AOC1, expression as N-terminally GST-tagged enzyme in Escherichia coli
AOC2, expression as N-terminally GST-tagged enzyme in Escherichia coli
cloning of the promoter of AaAOC, the key gene of jasmonate biosynthetic pathway, expression analysis, AaAOC gene overexpression under control of the 35S promoter in Artemisia annua leading to an increase of 1.6 to 5.2fold in enzyme expression and 2 to 4.7fold increase in jasmonate levels, as well as to a significant increase of contents of artemisinin, dihydroartemisinic acid and artemisinic acid, expression analysis by RT-Q-PCR
-
expressed in bread wheat and Arabidopsis thaliana
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli M15 cells
-
expressed in Escherichia coli SG13009 pRep4 cells
-
gene OsAOC, OsAOC lacking the chloroplast transit peptide is amplified from cultivar Nihonmasari cDNA and expressed as GST-tagged enzyme in Escherichia coli strain BL21
Q8L6H4
generating of a jasmonic acid-deficient rice line with suppressed expression of the genes encoding AOC with resistance to Magnaporthe grisea by introduction into rice plants by Agrobacterium-mediated transformation, the expression of AOC is induced after jasmonic acid and wound treatment, AOC-interfering RNA plants show normal resistance to both an incompatible and a compatible race of Magnaporthe grisea
Q8L6H4
into the vector pGEM-T Easy for sequencing and subsequently into the vector pQE30 for expression in Escherichia coli M15 cells
into the vector pMD18-T and for expression in Escherichia coli BL21DE3 cells subsequently into the vector pET-30a
transfection into tobacco via Agrobacterium tumefaciens