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5.3.99.5: thromboxane-A synthase

This is an abbreviated version!
For detailed information about thromboxane-A synthase, go to the full flat file.

Word Map on EC 5.3.99.5

Reaction

(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
=
(5Z,13E)-(15S)-9alpha,11alpha-epoxy-15-hydroxythromboxa-5,13-dienoate

Synonyms

CYP5A1, Synthetase, thromboxane, TBXAS1, Thromboxan synthetase, thromboxane A synthase 1, thromboxane A-synthase, thromboxane A2 synthase, thromboxane A2 synthase 1, Thromboxane A2 synthetase, thromboxane synthase, Thromboxane synthetase, TXA synthase, TxA-synthase, TXA2 synthase, TXAS, TXS, TXSA, TxSI

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.99 Other intramolecular oxidoreductases
                5.3.99.5 thromboxane-A synthase

Purification

Purification on EC 5.3.99.5 - thromboxane-A synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
immunoaffinity purification
-
intrinsic membrane protein, detergent treatment is necessary
-
one-step immunoaffinity purification
-
partial
recombinant C-terminally His-tagged modified enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography
-
recombinant His-tagged TXAS
-
recombinant TXAS
-
recombinant TXAS, Ni-NTA, column, DEAE-column, Octyl-column
-
recombinant wild-type and mutant C-terminally His4-tagged modified enzyme from Escherichia coli strain BL21/(DE3)pLys by nickel affinity and hydroxyapatite chromatography
-