Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

5.3.99.2: Prostaglandin-D synthase

This is an abbreviated version!
For detailed information about Prostaglandin-D synthase, go to the full flat file.

Word Map on EC 5.3.99.2

Reaction

(5Z,13E,15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
=
(5Z,13E,15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate

Synonyms

beta-Trace, Beta-trace protein, betaTP, glutathione dependent prostaglandine D2 synthase, Glutathione-dependent PGD synthetase, glutathione-dependent prostaglandin D2 synthase, Glutathione-independent PGD synthetase, H-PGDS, haematopoietic PGD synthase, haematopoietic prostaglandin D synthase, Hematopoietic prostaglandin D synthase, hematopoietic prostaglandin D2 synthase, HPGDS, Isomerase, prostaglanin R2 D-, L-PGDS, L-prostaglandin D synthase, L-type prostaglandin synthase, lipocalin prostaglandin D synthase, lipocalin type prostaglandin D synthase, lipocalin-prostaglandin D synthase, lipocalin-type PG D synthase, lipocalin-type PGD synthase, lipocalin-type Pgds, lipocalin-type prostaglandin d synthase, lipocalin-type prostaglandin D2 synthase, lipocaline-type prostaglandin D synthase, PGD synthase, PGD-S, PGD2 synthase, PGD2 synthetase, PGDS, PGDS2, PGH-PGD isomerase, PGH2 D-isomerase, Prostaglandin D synthase, Prostaglandin D2 synthase, prostaglandin D2 synthetase, prostaglandin synthase, Prostaglandin-D synthase, Prostaglandin-H2 D-isomerase, Prostaglandin-R-prostaglandin D isomerase, PTGDS

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.99 Other intramolecular oxidoreductases
                5.3.99.2 Prostaglandin-D synthase

Cloned

Cloned on EC 5.3.99.2 - Prostaglandin-D synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in 3T3-L1 cells
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli C41(DE3) cells
expressed in Escherichia coli Rosetta (DE3) pLysS cells
expressed in Escherichia coli XL-1 Blue cells
expressed in Medicago truncatula cells and Nicotiana tabacum leaves
expression as glutathione S-transferase fusion protein in Escherichia coli
-
expression in Escherichia coli
expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta BL21(DE3)
expression of cDNA in Escherichia coli
expression of GST-tagged enzyme mutant C65A/C167A, with truncated N-terminal 22-amino acid residues corresponding to the putative secretion signal peptide, in Escherichia coli strain BL21(DE3)
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
for expression in Escherichia coli BL21DE3 cells
for expression in Escherichia coli cells
into the vector pET17b for expression in Escherichia coli BL21DE3 cells
into the vector pT7-7 for expression in Escherichia coli BL21DE3 cells
into the vectors pUB6/V5-His and pIRESneo, cDNA of positions 76-648 is cloned into the vector pGEM-T Easy
-
mutant C65A is expressed in Escherichia coli BL21(DE3) cells
the coding region of human L-PGDS is sub-cloned into pCR-Script Amp SK+ vector
-