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5.3.4.1: protein disulfide-isomerase

This is an abbreviated version!
For detailed information about protein disulfide-isomerase, go to the full flat file.

Word Map on EC 5.3.4.1

Reaction

catalyses the rearrangement of -S-S- bonds in proteins =

Synonyms

5'-MD, 58 kDa glucose regulated protein, 58 kDa microsomal protein, AGR2, anterior gradient homolog 2, BPA-binding protein, CaBP1, CaBP2, Cellular thyroid hormone binding protein, cotyledon-specific chloroplast biogenesis factor CYO1, CYO1, DbsG, disulfide bond isomerase, disulfide bond-forming enzyme, Disulfide interchange enzyme, disulfide isomerase, Disulfide isomerase ER-60, disulfide-bond isomerase, dithiol-disulfide isomerase, Dsb, DsbA, DsbB, DsbC, DsbD, DsbG, ECaSt/PDI, endoplasmic reticulum protein EUG1, Eps1p, ER protein 57, ER58, ER60, ERcalcistorin/protein-disulfide isomerase, ERdj5, Ero1, Erp, ERP-57, ERp-72 homolog, ERp18, ERp27, ERp28, ERp44, Erp46, ERp5, ERp57, ERP59, ERP60, ERp72, Eug1p, fibronectin, gPDI-1, gPDI-2, gPDI-3, HIP-70, HlPDI-1, HlPDI-2, HlPDI-3, Iodothyronine 5'-monodeiodinase, More, Mpd1p, Mpd2p, multifunctional protein disulfide isomerase, ncgl2478, P5, P55, P58, pancreas-specific protein disulfide isomerase, PDI, PDI A4, PDI I, PDI II, pdi-15, PDI-1a, pdi-40, pdi-47, pdi-52, PDI-A, PDI-M, PDI-P5, PDI-related protein, PDI1, PDI11, PDI2, PDI7, PDI8, PDIA1, PDIA2, PDIA3, PDIA4, PDIA6, PDIL-1, PDIL-2, PDIL1-1, PDIL1;1, PDIL1Aalpha, PDIL1B, PDIL2, PDIL2-3, PDIL3A, PDIL4D, PDIL5A, PDILT, PDIp, PDIr, protein disulfide isomerase, protein disulfide isomerase 1, protein disulfide isomerase 2, protein disulfide isomerase 3, protein disulfide isomerase A1, protein disulfide isomerase A3, protein disulfide isomerase A5, protein disulfide isomerase A6, protein disulfide isomerase associated 3, Protein disulfide isomerase P5, protein disulfide isomerase-1, protein disulfide isomerase-11, protein disulfide isomerase-2, protein disulfide isomerase-3, protein disulfide isomerase-8, protein disulfide isomerase-like protein of the testis, protein disulfide isomerase-P5, protein disulfide isomerase-related chaperone Wind, Protein disulfide isomerase-related protein, protein disulfide oxidoreductase, protein disulfide reductase/isomerase, protein disulfide-isomerase A4, Protein disulphide isomerase, Protein ERp-72, protein-disulfide isomerase, R-cognin, RB60, Rearrangease, Reduced ribonuclease reactivating enzyme, Retina cognin, S-S rearrangase, SSO0192, SsPDO, thiol-disulfide oxidoreductase, thiol-protein oxidoreductase, thioredoxin domain-containing protein 5, Thyroid hormone-binding protein, Thyroxine deiodinase, TXNDC5, yPDI

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.4 Transposing S-S bonds
                5.3.4.1 protein disulfide-isomerase

Molecular Weight

Molecular Weight on EC 5.3.4.1 - protein disulfide-isomerase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
gel filtration
107000
108000
110000
114800
-
gel filtration
116000
gel filtration, the C-terminal fragment comprising residues 441-491 contributes to the anomalous molecular mass determination by gel filtration
120000
122000
-
gel filtration
12650
gPDI-3, deduced from nucleotide sequence
130000 - 300000
-
GmPDIL-3a-GmPDIL-3b protein complexes
140000
15000
x * 15000, isozyme pdi-15, SDS-PAGE
17770
-
MALDI mass spectrometry
208000
PDIL2-3, gel filtration
21100
22000
-
2 * 22000, DsbA, SDS-PAGE, 2 * 25000, DsbC, SDS-PAGE
25000
-
2 * 22000, DsbA, SDS-PAGE, 2 * 25000, DsbC, SDS-PAGE
25850
gPDI-1, deduced from nucleotide sequence
25910
-
x * 25910, calculation from nucleotide sequence
26032
x * 26032, calculated from sequence
27100
gel filtration, electrospray mass spectrometry
27300
-
x * 27300, calculated from amino acid sequence
27700
x * 27700, ERp27, SDS-PAGE
30000
-
x * 30000, His6-tagged enzyme, SDS-PAGE
326000
-
gel filtration, in presence of 1 mM Zn2+
39490
x * 39490, sequece calculation
40000
x * 40000, isozyme pdi-40, SDS-PAGE
45000
x * 45000, recombinant PDI-1, SDS-PAGE, x * 80000, recombinant PDI-2, SDS-PAGE
47000
x * 47000, isozyme pdi-47, SDS-PAGE
47850
x * 47850, sequence calculation
48074
x * 48074, PDI-P5, sequence calculation, x * 56859, PDIA3, sequence calculation
50430
52000
53000
-
x * 53000, SDS-PAGE
53100
-
x * 53100, oxidized enzyme, calculated from amino acid sequence
53680
x * 53680, sequence calculation
54000
-
x * 54000, ERP-57
54300
-
x * 54300, calculation from nucleotide sequence
54890
x * 54890, sequence calculation
55114
-
x * 55114, calculation from nucleotide sequence
56000
56300
-
1 * 56300, calculated from amino acid sequence
56400
-
x * 56400, calculated from amino acid sequence
56859
x * 48074, PDI-P5, sequence calculation, x * 56859, PDIA3, sequence calculation
57000
57700
-
1 * 57700, analytical ultracentrifugation
58000
58800
-
x * 58800, reduced enzyme, small-angle X-ray scattering
58900
-
x * 58900, SDS-PAGE under reducing conditions, other bands with MW 53800 and 55200 represent proteolytic degradation products of the 58900 MW protein
60000
61200
-
x * 61200, SDS-PAGE under reducing conditions
62000
63000
64000
1 * 64000, analytical ultracentrifugation
65000
66400
-
x * 66400, oxidized enzyme, small-angle X-ray scattering
69000
light scattering
70000
-
2 * 70000, SDS-PAGE
72000
-
x * 72000, SDS-PAGE
72397
4 * 72397, PDIL2-3, calculated from amino acid sequence
80000
x * 45000, recombinant PDI-1, SDS-PAGE, x * 80000, recombinant PDI-2, SDS-PAGE
85000
x * 55000, recombinant PDI, SDS-PAGE, x * 85000, recombinant GST-tagged PDI, SDS-PAGE
additional information
-
Zn2+ induces formation of larger enzyme aggregates with MW up to 600 kDa and above