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5.3.3.8: DELTA3-DELTA2-enoyl-CoA isomerase

This is an abbreviated version!
For detailed information about DELTA3-DELTA2-enoyl-CoA isomerase, go to the full flat file.

Word Map on EC 5.3.3.8

Reaction

a (3E)-alk-3-enoyl-CoA
=
a (2E)-alk-2-enoyl-CoA

Synonyms

2,3-Enoyl-CoA isomerase, 2,3-trans-enoyl-CoA isomerase, 3,2-trans-Enoyl-CoA isomerase, 3,2-trans-enoyl-coenzyme A isomerase, 3-2trans-Enoyl-CoA isomerase, 3-cis-2-trans-Enoyl-CoA isomerase, Acetylene-allene isomerase, AtECI1, AtECI2, AtECI3, D3,D2-enoyl-CoA isomerase, DCI, DELTA3,DELTA2-enoyl CoA isomerase, DELTA3,DELTA2-enoyl-CoA isomerase, DELTA3-cis,DELTA2-trans-Enoyl-CoA isomerase, DELTA3-cis-DELTA2-trans-enoyl-CoA isomerase, Delta3-Delta2-enoyl-CoA isomerase, DELTA3DELTA2-enoyl CoA isomerase, dodecenoyl coenzyme A DELTA isomerase, Dodecenoyl-CoA delta-isomerase, dodecenoyl-CoA DELTA3-cis-DELTA2-trans-isomerase, dodecenoyl-coenzyme A DELTA isomerase, ECI, ECI1, Eci1p, ECI2, Eci3, Hepatocellular carcinoma-associated antigen 88, Isomerase, dodecenoyl coenzyme A Delta-, Long-chain DELTA3,DELTA2-enoyl-CoA isomerase, MECI, MFE1, PECI, Short chain DELTA3,DELTA2-enoyl-CoA isomerase

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.3 Transposing C=C bonds
                5.3.3.8 DELTA3-DELTA2-enoyl-CoA isomerase

Engineering

Engineering on EC 5.3.3.8 - DELTA3-DELTA2-enoyl-CoA isomerase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E134Q
-
catalytic properties of the five different component enzyme activities of the alpha/Arg134Gln mutant complex show no significant changes as compared with those of the wild-type complex. In the alpha/Glu139Gln mutant complex DELTA3-cis-DELTA2-trans-enoyl-CoA isomerase shows 60% decreased turnover number and a significant increase in Km for 3-cis-tetradecenoyl-CoA
E139Q
-
The replacement of Glu165 by Gln leads to a strongly reduced enzymatic activity. Tyr150 is not involved in isomerase catalysis
E136A
variant to study the importance of Glu136 for catalysis
D149E
E151X
-
site-directe mutagenesis of putative active-site amino acid residues. Exchange of Arg151 and Asp211 leads to a reduced expression of the recombinant enzyme accompanied by a reduced activity. The replacement of Glu165 by Gln leads to a strongly reduced enzymatic activity. Tyr150 is not involved in isomerase catalysis
E165Q
-
site-directe mutagenesis of putative active-site amino acid residues. Exchange of Arg151 and Asp211 leads to a reduced expression of the recombinant enzyme accompanied by a reduced activity. The replacement of Glu165 by Gln leads to a strongly reduced enzymatic activity. Tyr150 is not involved in isomerase catalysis
K242C
K242D
K242F
K242R
K242T
N211X
-
site-directe mutagenesis of putative active-site amino acid residues. Exchange of Arg151 and Asp211 leads to a reduced expression of the recombinant enzyme accompanied by a reduced activity. The replacement of Glu165 by Gln leads to a strongly reduced enzymatic activity. Tyr150 is not involved in isomerase catalysis
F268A
the mutant shows reduced activity compared to the wild type enzyme
N101A
the mutant shows reduced activity compared to the wild type enzyme
R100A
the mutant shows reduced activity compared to the wild type enzyme
additional information
-
in the absence of DELTA3,DELTA2-enoyl CoA isomerase, complete beta-oxidation of (10Z)-heptadecanoic acid is blocked. In vivo enzyme activity tests of the proteins encoded by AtECI1, AtECI2, and AtECI3 by complementation of the Saccharomyces cerevisiae double mutant eciDELTAdci1DELTA with deletions of the genes, DELTA2,DELTA3-enoyl-CoA isomerase and DELTA3,4,DELTA2,4-dienoyl CoA isomerase