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0.017
dimethylallyl diphosphate
Cinchona robusta
-
isomerase I
0.0008 - 15.3
isopentenyl diphosphate
additional information
additional information
-
substrate binds to the inactive oxidized and active reduced form of enzyme with similar affinities
-
0.0008
isopentenyl diphosphate
mutant enzyme W161F
0.001
isopentenyl diphosphate
Cinchona robusta
-
isomerase II
0.0013
isopentenyl diphosphate
-
isomerase II and III
0.0015
isopentenyl diphosphate
pH 6.0, 60°C, mutant enzyme Q160L
0.00152
isopentenyl diphosphate
mutant enzyme Q160L, in 50 mM MOPS-NaOH, pH 6.0, at 60°C
0.0017
isopentenyl diphosphate
-
isomerase I
0.0025
isopentenyl diphosphate
-
isomerase IV
0.0027
isopentenyl diphosphate
-
-
0.003
isopentenyl diphosphate
mutant enzyme R83K
0.0032
isopentenyl diphosphate
pH 6.0, 60°C, mutant enzyme Q160N
0.00323
isopentenyl diphosphate
mutant enzyme Q160N, in 50 mM MOPS-NaOH, pH 6.0, at 60°C
0.0033
isopentenyl diphosphate
-
wild type enzyme, in 25 mM Tris-HCl, pH 7.0, at 37°C
0.0035
isopentenyl diphosphate
wild-type enzyme
0.004
isopentenyl diphosphate
-
-
0.0048
isopentenyl diphosphate
-
aerobic, presence of NADPH, pH 7.0, 37°C
0.0051
isopentenyl diphosphate
Cinchona robusta
-
isomerase I
0.0054
isopentenyl diphosphate
-
-
0.0056
isopentenyl diphosphate
-
pH 7.0, 37°C
0.0057
isopentenyl diphosphate
-
-
0.00595
isopentenyl diphosphate
-
wild type enzyme, at anaerobic conditions, at pH 7.0 and 37°C
0.006
isopentenyl diphosphate
-
-
0.006
isopentenyl diphosphate
-
mutant enzyme E169Q, in 25 mM Tris-HCl, pH 7.0, at 37°C
0.006
isopentenyl diphosphate
-
mutant enzyme Y105F, in 25 mM Tris-HCl, pH 7.0, at 37°C
0.0071
isopentenyl diphosphate
pH 6.0, 60°C, mutant enzyme Q160H
0.00713
isopentenyl diphosphate
mutant enzyme Q160H, in 50 mM MOPS-NaOH, pH 6.0, at 60°C
0.00714
isopentenyl diphosphate
pH 6.0, 60°C, mutant enzyme E13R/R235E
0.00739
isopentenyl diphosphate
wild type enzyme, in 50 mM MOPS-NaOH, pH 6.0, at 60°C
0.0074
isopentenyl diphosphate
pH 6.0, 60°C, wild-type enzyme
0.0076
isopentenyl diphosphate
-
-
0.00826
isopentenyl diphosphate
mutant enzyme Q160E, in 50 mM MOPS-NaOH, pH 6.0, at 60°C
0.0083
isopentenyl diphosphate
pH 6.0, 60°C, mutant enzyme Q160E
0.0095
isopentenyl diphosphate
wild-type, pH 7.4, 37°C
0.0115
isopentenyl diphosphate
mutant enzyme E87Q
0.0116
isopentenyl diphosphate
-
mutant enzyme W216F, in 25 mM Tris-HCl, pH 7.0, at 37°C
0.012
isopentenyl diphosphate
-
aerobic, presence of dithionite, pH 7.0, 37°C
0.0128
isopentenyl diphosphate
-
mutant enzyme Y159F, in 25 mM Tris-HCl, pH 7.0, at 37°C
0.014
isopentenyl diphosphate
mutant enzyme K55A
0.0142
isopentenyl diphosphate
mutant Y104F, pH 7.4, 37°C
0.015
isopentenyl diphosphate
mutant enzyme K55R
0.0168
isopentenyl diphosphate
-
anaerobic, presence of NADPH, pH 7.0, 37°C
0.0185
isopentenyl diphosphate
mutant enzyme R51K
0.02
isopentenyl diphosphate
-
-
0.021
isopentenyl diphosphate
mutant enzyme E116Q
0.0225
isopentenyl diphosphate
mutant Y104A, pH 7.4, 37°C
0.0228
isopentenyl diphosphate
-
pH 8.0
0.033
isopentenyl diphosphate
-
-
0.035
isopentenyl diphosphate
-
-
0.036
isopentenyl diphosphate
-
-
0.0376
isopentenyl diphosphate
mutant enzyme L141H/Y195F/W256C, at pH 7.5 and 30°C
0.04
isopentenyl diphosphate
at pH 7.0 and 37°C
0.0408
isopentenyl diphosphate
-
wild type enzyme, at aerobic conditions, at pH 7.0 and 37°C
0.0415
isopentenyl diphosphate
wild type enzyme, at pH 7.5 and 30°C
0.043
isopentenyl diphosphate
-
-
0.046
isopentenyl diphosphate
-
pH 8.0, 50°C
0.0463
isopentenyl diphosphate
pH 6.0, 60°C, wild-type enzyme
0.047
isopentenyl diphosphate
-
pH 8.0, 55°C
0.063
isopentenyl diphosphate
pH 6.0, 60°C
0.063
isopentenyl diphosphate
-
pH 8.0, 60°C
0.064
isopentenyl diphosphate
-
-
0.065
isopentenyl diphosphate
-
pH 8.0, 70°C
0.071
isopentenyl diphosphate
-
pH 8.0, 65°C
0.077
isopentenyl diphosphate
-
pH 8.0, 75°C
0.084
isopentenyl diphosphate
-
pH 8.0, 80°C
0.0841
isopentenyl diphosphate
-
pH 6.0
0.093
isopentenyl diphosphate
-
pH 8.0, 90°C
0.187
isopentenyl diphosphate
-
pH 8.0, 82.5°C
0.199
isopentenyl diphosphate
-
pH 8.0, 87.5°C
0.211
isopentenyl diphosphate
-
pH 8.0, 85°C
0.2768
isopentenyl diphosphate
-
in 0.4 M Tris, 1 mM dithiothreitol, 10 mM MgCl2, pH 8.0, at 50°C
0.572
isopentenyl diphosphate
-
mutant enzyme N37A, at aerobic conditions, at pH 7.0 and 37°C
15.3
isopentenyl diphosphate
-
pH 7.0, 85°C
0.0874
NADH
dehydrogenase reaction without isopentenyl diphosphate
0.0904
NADH
isomerase reaction