5.3.3.17: trans-2,3-dihydro-3-hydroxyanthranilate isomerase
This is an abbreviated version!
For detailed information about trans-2,3-dihydro-3-hydroxyanthranilate isomerase, go to the full flat file.
Reaction
Synonyms
phzF
ECTree
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Crystallization
Crystallization on EC 5.3.3.17 - trans-2,3-dihydro-3-hydroxyanthranilate isomerase
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1.8 A crystal structure of PhzF, hanging drop vapor diffusion method
hanging-drop vapor-diffusion method. Crystals of unliganded PhzF belong to space group P3(2)21 with a a = b = 56 A, and c = 156 A and hold one monomer per asymmetric unit. Complexes of PhzF (with 3-hydroxyanthranilic acid and trans-2,3-dihydro-3-hydroxyanthranilic acid) crystallize in space group P2(1)2(1)2 with cell parameters of a = 93 A, b = 100 A, and c = 57 A and contain one dimer in the asymmetric unit
hanging-drop vapour-diffusion method. Crystallized from PEG 4000/ammonium sulfate/sodium citrate pH 5.6. The crystals belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 56.3, c = 156.4 A. They contain one monomer in the asymmetric unit and diffract to better than 1.7 A on synchrotron beamlines. Crystals of seleno-L-methionine-labelled PhzF have been obtained