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5.3.2.3: TDP-4-oxo-6-deoxy-alpha-D-glucose-3,4-oxoisomerase (dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose-forming)

This is an abbreviated version!
For detailed information about TDP-4-oxo-6-deoxy-alpha-D-glucose-3,4-oxoisomerase (dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose-forming), go to the full flat file.

Word Map on EC 5.3.2.3

Reaction

dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
=
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose

Synonyms

dTDP-6-deoxy-D-xylo-hexos-4-ulose 3,4-ketoisomerase, FdtA, RavIM, TDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.2 Interconverting keto- and enol-groups
                5.3.2.3 TDP-4-oxo-6-deoxy-alpha-D-glucose-3,4-oxoisomerase (dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose-forming)

Crystallization

Crystallization on EC 5.3.2.3 - TDP-4-oxo-6-deoxy-alpha-D-glucose-3,4-oxoisomerase (dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose-forming)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystallization of wild-type and selenomethionine-labeled FdtA, crystallization of the mutant proteins (H49N, H51N, H49N/H51N), hanging-drop method of vapor diffusion, crystallization trials are conducted at both 25°C and 4°C. Three-dimensional structure of this sugar isomerase complexed with dTDP and solved to 1.5 A resolution. Crystals belong to the space group P4(1)2(1)2 with unit cell dimensions of a = b = 62.7 A and c = 201.3 A and one dimer per asymmetric unit
hanging drop vapor diffusion method, using 20% poly(ethylene glycol) 8000, 200 mM NaCl, and 100 mM MOPS (pH 7.0)