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2 - 10
D-Ribose 5-diphosphate
0.017 - 37
D-ribose 5-phosphate
0.52 - 4.41
D-ribose-5-phosphate
34
D-ribulose
-
in 50 mM Tris-HCl buffer (pH 7.5), at 65°C
1.4 - 2.5
D-ribulose 5-diphosphate
0.015 - 15
D-ribulose 5-phosphate
232
D-talose
-
in 50 mM Tris-HCl buffer (pH 7.5), at 65°C
98
L-allose
-
in 50 mM Tris-HCl buffer (pH 7.5), at 65°C
173
L-ribose
-
in 50 mM Tris-HCl buffer (pH 7.5), at 65°C
319
L-ribulose
-
in 50 mM Tris-HCl buffer (pH 7.5), at 65°C
125
L-tagatose
-
in 50 mM Tris-HCl buffer (pH 7.5), at 65°C
additional information
additional information
-
106
D-allose
-
in 50 mM Tris-HCl buffer (pH 7.5), at 65°C
130
D-allose
pH 8.0 and 70°C
460
D-allose
-
pH 7.5, 40°C
460
D-allose
AM180355
pH 7.5 and 40°C
43
D-psicose
mutant enzyme R132E, pH 7.5, 80°C
46
D-psicose
mutant enzyme R132D, pH 7.5, 80°C
53
D-psicose
-
in 50 mM Tris-HCl buffer (pH 7.5), at 65°C
53
D-psicose
mutant enzyme R132K, pH 7.5, 80°C
53
D-psicose
wild type enzyme, pH 7.5, 80°C
54
D-psicose
mutant enzyme R132Q, pH 7.5, 80°C
56
D-psicose
mutant enzyme R132A, pH 7.5, 80°C
64
D-psicose
-
at pH 8.0 and 75°C
78
D-psicose
mutant enzyme R132I, pH 7.5, 80°C
44
D-ribose
-
in 50 mM Tris-HCl buffer (pH 7.5), at 65°C
110
D-ribose
pH 8.0 and 70°C
270
D-ribose
-
pH 7.5, 40°C
270
D-ribose
AM180355
pH 7.5 and 40°C
2
D-Ribose 5-diphosphate
mutant H102A, pH 8.4, 25°C
2 - 3
D-Ribose 5-diphosphate
muant H11A, pH 8.4, 25°C
4
D-Ribose 5-diphosphate
wild-type, pH 8.4, 25°C
10
D-Ribose 5-diphosphate
mutant H138A, pH 8.4, 25°C
0.017
D-ribose 5-phosphate
-
pH 7.5, 65°C, recombinant enzyme
0.108
D-ribose 5-phosphate
-
-
0.16
D-ribose 5-phosphate
-
enzyme form III
0.17
D-ribose 5-phosphate
50°C, pH 6.0, mutant enzyme D85N
0.43
D-ribose 5-phosphate
-
-
0.67
D-ribose 5-phosphate
-
enzyme form II
0.74
D-ribose 5-phosphate
-
-
0.77
D-ribose 5-phosphate
-
enzyme form I
0.83
D-ribose 5-phosphate
-
ribosephosphate isomerase B
0.88
D-ribose 5-phosphate
-
-
1
D-ribose 5-phosphate
in 50 mM Tris-HCl, pH 7.5, at 37°C
1 - 2.1
D-ribose 5-phosphate
-
at 80°C and pH 7.0
1.17
D-ribose 5-phosphate
50°C, pH 6.0, wild-type enzyme
1.63
D-ribose 5-phosphate
-
-
1.8
D-ribose 5-phosphate
-
-
1.8
D-ribose 5-phosphate
-
2
D-ribose 5-phosphate
-
-
2.2
D-ribose 5-phosphate
-
-
2.39
D-ribose 5-phosphate
50°C, pH 6.0, mutant enzyme D168N
2.5
D-ribose 5-phosphate
-
-
2.51
D-ribose 5-phosphate
wild-type, pH 7.5, 22°C
2.59
D-ribose 5-phosphate
mutant Y46F, pH 7.5, 22°C
2.6
D-ribose 5-phosphate
-
calf spleen
2.7
D-ribose 5-phosphate
-
-
2.8
D-ribose 5-phosphate
-
-
3.1
D-ribose 5-phosphate
-
3.3
D-ribose 5-phosphate
-
-
3.4
D-ribose 5-phosphate
mutant H11N, pH 7.5, 22°C
3.7
D-ribose 5-phosphate
-
-
4.3
D-ribose 5-phosphate
mutant P47A, pH 7.5, 22°C
4.4
D-ribose 5-phosphate
-
ribosephosphate isomerase A
4.6
D-ribose 5-phosphate
-
-
5.1
D-ribose 5-phosphate
50°C, pH 6.0, mutant enzyme K125A
6.5
D-ribose 5-phosphate
-
ox muscle
7.13
D-ribose 5-phosphate
50°C, pH 6.0, mutant enzyme R100A
7.6
D-ribose 5-phosphate
-
at 60°C and pH 7.0
8.14
D-ribose 5-phosphate
mutant H138N, pH 7.5, 22°C
9.1 - 10.2
D-ribose 5-phosphate
-
calf liver
17
D-ribose 5-phosphate
-
in 50 mM Tris-HCl buffer (pH 7.5), at 65°C
30
D-ribose 5-phosphate
-
pH 7.5, 40°C
30
D-ribose 5-phosphate
AM180355
pH 7.5 and 40°C
37
D-ribose 5-phosphate
pH 8.0 and 70°C
0.52
D-ribose-5-phosphate
-
pH 8.0, 25°C, mutant enzyme D90A
0.56
D-ribose-5-phosphate
-
pH 8.0, 25°C, mutant enzyme D87A
0.63
D-ribose-5-phosphate
-
pH 8.0, 25°C, wild-type enzyme
1.17
D-ribose-5-phosphate
-
pH 8.0, 25°C, mutant enzyme E91A
4.41
D-ribose-5-phosphate
-
pH 8.0, 25°C, mutant enzyme K100A
1.4
D-ribulose 5-diphosphate
wild-type, pH 8.4, 25°C
2.5
D-ribulose 5-diphosphate
mutant H102A, pH 8.4, 25°C
0.015
D-ribulose 5-phosphate
-
pH 7.5, 65°C, recombinant enzyme
0.66
D-ribulose 5-phosphate
-
-
5
D-ribulose 5-phosphate
in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C
15
D-ribulose 5-phosphate
-
in 50 mM Tris-HCl buffer (pH 7.5), at 65°C
5 - 20
L-talose
-
pH 7.5, 40°C
5 - 20
L-talose
AM180355
pH 7.5 and 40°C
37
L-talose
-
in 50 mM Tris-HCl buffer (pH 7.5), at 65°C
190
L-talose
pH 8.0 and 70°C
additional information
additional information
dramatic increase of Km-value at temperatures above 80°C
-
additional information
additional information
-
dramatic increase of Km-value at temperatures above 80°C
-
additional information
additional information
-
kinetics with aldose and ketose substrates, recombinant enzyme, overview
-