5.3.1.32: (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase

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For detailed information about (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, go to the full flat file.

Reaction

(4S)-4-hydroxy-5-phosphooxypentane-2,3-dione

Synonyms

lsrG, phospho-AI-2 isomerase

ECTree

5 Isomerases

5.3 Intramolecular oxidoreductases

5.3.1 Interconverting aldoses and ketoses, and related compounds

5.3.1.32 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase

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Results	in table
937	AA Sequence
1	Cloned(Commentary)
1	Crystallization (Commentary)
6	General Information
1	Inhibitors
4	Natural Substrates/ Products (Substrates)
4	Organism
1	Pathway
1	pH Optimum
8	Protein Variants
1	Purification (Commentary)
1	Reaction
4	Reference
3	Specific Activity [micromol/min/mg]
8	Substrates and Products (Substrate)
8	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Engineering

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Engineering on EC 5.3.1.32 - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase

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E54A

Escherichia coli O55:H7

D3QT67

no activity

732039

H65A

Escherichia coli O55:H7

D3QT67

no activity

732039

H70A

Escherichia coli O55:H7

D3QT67

specific activity is about 1% compared to wild-type activity

732039

N25A

Escherichia coli O55:H7

D3QT67

specific activity is about 35% compared to wild-type activity

732039

E54A

Escherichia coli O55:H7 CB9615

no activity

H65A

Escherichia coli O55:H7 CB9615

no activity

H70A

Escherichia coli O55:H7 CB9615

specific activity is about 1% compared to wild-type activity

N25A

Escherichia coli O55:H7 CB9615

specific activity is about 35% compared to wild-type activity