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5.3.1.24: phosphoribosylanthranilate isomerase

This is an abbreviated version!
For detailed information about phosphoribosylanthranilate isomerase, go to the full flat file.

Word Map on EC 5.3.1.24

Reaction

N-(5-phospho-beta-D-ribosyl)anthranilate
=
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate

Synonyms

IGPS:PRAI (indole-3-glycerol-phosphate synthetase/N-5'-phosphoribosylanthranilate isomerase complex), isomerase, phosphoribosylanthranilate, More, N-(5'-phosphoribosyl)anthranilate isomerase, N-(5'-phosphoribosyl)anthranilate isomerase/dehydratase, N-(5-phospho-beta-D-ribosyl)anthranilate ketol-isomerase, N-5’-phosphoribosylanthranilate isomerase, PAI, PfTrpF, phosphoribosyl anthranilate isomerase, phosphoribosyl isomerase A, phosphoribosylanthranilate isomerase, PRA isomerase, PRAI, PRAI-LoxP, PRAI[ML256-452], PriA, PurF(I198V), Trp1, Trp1p, TrpF, TrpFCtL2, TtPRAI, xPRAI

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.1 Interconverting aldoses and ketoses, and related compounds
                5.3.1.24 phosphoribosylanthranilate isomerase

Purification

Purification on EC 5.3.1.24 - phosphoribosylanthranilate isomerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a nickel Sepharose column, HisTrap FF, and a gel-filtration column, Sephacryl S200, are used
best isolated from mutant trp A2
-
component IIb of the anthranilate synthetase complex, purified from trp2 mutant
-
HisTrap FF nickel affinity column chromatography
-
indole-3-glycerol-phosphate synthetase/PRA isomerase complex
-
indole-3-glycerol-phosphate synthetase/PRA isomerase complex, 70fold
-
indole-3-glycerol-phosphate synthetase/PRA isomerase fragment of the multifunctional anthranilate synthetase complex beta subunit
-
monomeric domain PRAI[ML256-452] and bifunctional IGPS:PRAI
-
mutationally generated monomeric PRA isomerase
-
Ni-NTA agarose column chromatography and Superdex 75 gel filtration
Ni-NTA column chromatography
-
Ni-NTA column chromatography, HiTrap Q column chromatography, Superdex 200 gel filtration
-
partial
recombinant PRAI, expressed in Escherichia coli
-
recombinant xPRAI, expressed in Escherichia coli
-
TrpF mutants C7A, D126N and C7A/D126N double mutant