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5.3.1.24: phosphoribosylanthranilate isomerase

This is an abbreviated version!
For detailed information about phosphoribosylanthranilate isomerase, go to the full flat file.

Word Map on EC 5.3.1.24

Reaction

N-(5-phospho-beta-D-ribosyl)anthranilate
=
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate

Synonyms

IGPS:PRAI (indole-3-glycerol-phosphate synthetase/N-5'-phosphoribosylanthranilate isomerase complex), isomerase, phosphoribosylanthranilate, More, N-(5'-phosphoribosyl)anthranilate isomerase, N-(5'-phosphoribosyl)anthranilate isomerase/dehydratase, N-(5-phospho-beta-D-ribosyl)anthranilate ketol-isomerase, N-5’-phosphoribosylanthranilate isomerase, PAI, PfTrpF, phosphoribosyl anthranilate isomerase, phosphoribosyl isomerase A, phosphoribosylanthranilate isomerase, PRA isomerase, PRAI, PRAI-LoxP, PRAI[ML256-452], PriA, PurF(I198V), Trp1, Trp1p, TrpF, TrpFCtL2, TtPRAI, xPRAI

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.1 Interconverting aldoses and ketoses, and related compounds
                5.3.1.24 phosphoribosylanthranilate isomerase

Cloned

Cloned on EC 5.3.1.24 - phosphoribosylanthranilate isomerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of trpF mutants, expression in Escherichia coli BL21(DE3)
ecotype Columbia, 3 PAI isogenes: PAI1, PAI2 and PAI3
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli M15 (pREP4) cells
-
expressed in Escherichia coli XL1-Red cells
-
expression in Escherichia coli
into the vector pET-15b
overview over trp/TRP genes encoding PRAI, functional organization of enzymes of tryptophan biosynthesis
separate enzyme encoded by an independent gene, not associated with indole-3-glycerol-phosphate synthetase
-
separation of the 2 domains of the indole-3-glycerol-phosphate synthetase/PRA isomerase complex on the gene level, expression of monofunctional PRAI[ML256-452] in Escherichia coli W3110 trpR, deltatrp EA2
-
sequence of trpCF gene encoding indole-3-glycerol-phosphate synthetase/PRA isomerase complex
-
the expression of PRAI-LoxP variants is performed in Escherichia coli Rosetta 2 cells transformed with pET28b+ plasmids containing the encoding sequences
TRP1 gene encoding indole-3-glycerol-phosphate synthetase/PRA isomerase complex is cloned, expressed in Escherichia coli and sequenced
TRP1 gene encoding multifunctional enzyme is cloned in Escherichia coli and sequenced
-
TRP1 gene is cloned in Escherichia coli and sequenced
-
TRP1 gene regulation, cloning
-
TRPC gene encoding multifunctional enzyme is cloned in Escherichia coli and sequenced
-
trpDFEG gene cluster, trpF gene encodes PRA isomerase
-
trpF gene is cloned and expressed in Escherichia coli