5.3.1.24: phosphoribosylanthranilate isomerase
This is an abbreviated version!
For detailed information about phosphoribosylanthranilate isomerase, go to the full flat file.
Word Map on EC 5.3.1.24
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5.3.1.24
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indoleglycerol
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amidotransferase
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alpha8
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betaalpha8-barrels
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dual-substrate
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biotechnology
- 5.3.1.24
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indoleglycerol
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amidotransferase
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alpha8
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betaalpha8-barrels
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dual-substrate
- biotechnology
Reaction
Synonyms
IGPS:PRAI (indole-3-glycerol-phosphate synthetase/N-5'-phosphoribosylanthranilate isomerase complex), isomerase, phosphoribosylanthranilate, More, N-(5'-phosphoribosyl)anthranilate isomerase, N-(5'-phosphoribosyl)anthranilate isomerase/dehydratase, N-(5-phospho-beta-D-ribosyl)anthranilate ketol-isomerase, N-5’-phosphoribosylanthranilate isomerase, PAI, PfTrpF, phosphoribosyl anthranilate isomerase, phosphoribosyl isomerase A, phosphoribosylanthranilate isomerase, PRA isomerase, PRAI, PRAI-LoxP, PRAI[ML256-452], PriA, PurF(I198V), Trp1, Trp1p, TrpF, TrpFCtL2, TtPRAI, xPRAI
ECTree
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Cloned
Cloned on EC 5.3.1.24 - phosphoribosylanthranilate isomerase
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cloning of trpF mutants, expression in Escherichia coli BL21(DE3)
overview over trp/TRP genes encoding PRAI, functional organization of enzymes of tryptophan biosynthesis
separate enzyme encoded by an independent gene, not associated with indole-3-glycerol-phosphate synthetase
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separation of the 2 domains of the indole-3-glycerol-phosphate synthetase/PRA isomerase complex on the gene level, expression of monofunctional PRAI[ML256-452] in Escherichia coli W3110 trpR, deltatrp EA2
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sequence of trpCF gene encoding indole-3-glycerol-phosphate synthetase/PRA isomerase complex
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the expression of PRAI-LoxP variants is performed in Escherichia coli Rosetta 2 cells transformed with pET28b+ plasmids containing the encoding sequences
TRP1 gene encoding indole-3-glycerol-phosphate synthetase/PRA isomerase complex is cloned, expressed in Escherichia coli and sequenced
TRP1 gene encoding multifunctional enzyme is cloned in Escherichia coli and sequenced
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TRPC gene encoding multifunctional enzyme is cloned in Escherichia coli and sequenced
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trpF gene is cloned and expressed in Escherichia coli
overview over trp/TRP genes encoding PRAI, functional organization of enzymes of tryptophan biosynthesis
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overview over trp/TRP genes encoding PRAI, functional organization of enzymes of tryptophan biosynthesis
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overview over trp/TRP genes encoding PRAI, functional organization of enzymes of tryptophan biosynthesis
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overview over trp/TRP genes encoding PRAI, functional organization of enzymes of tryptophan biosynthesis
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overview over trp/TRP genes encoding PRAI, functional organization of enzymes of tryptophan biosynthesis
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overview over trp/TRP genes encoding PRAI, functional organization of enzymes of tryptophan biosynthesis
-