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5.3.1.1: triose-phosphate isomerase

This is an abbreviated version!
For detailed information about triose-phosphate isomerase, go to the full flat file.

Word Map on EC 5.3.1.1

Reaction

D-glyceraldehyde 3-phosphate
=
glycerone phosphate

Synonyms

CP 25, CTIMC, cTPI, cytoplasmic TPI, cytoplasmic triosephosphate isomerase, cytoTPI, D-glyceraldehyde-3-phosphate ketol-isomerase, GlTIM, Isomerase, triose phosphate, Lactacin B inducer protein, monoTIM, PfTIM, PfuTIM, Phosphotriose isomerase, plastidic TPI, plastidic triosephosphate isomerase, pTPI, SSO2592, TcTIM, TIM, TIM1, TIM2, TonTIM, TpI, TPI1, TpiA, Triose phosphate isomerase, Triose phosphate mutase, Triose phosphoisomerase, Triosephosphate isomerase, Triosephosphate mutase, vTIM

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.1 Interconverting aldoses and ketoses, and related compounds
                5.3.1.1 triose-phosphate isomerase

Temperature Stability

Temperature Stability on EC 5.3.1.1 - triose-phosphate isomerase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
half-life of wild-type enzyme: 58 min
104
half-life: 280 min
22
-
pH 7, stable indefinitely
25 - 60
-
the recombinant enzyme is stable at 25°C for 30 min. The recombinant enzyme retains 92.7%, 40% and 13% activity when preincubated at 37°C, 42°C and 60°C for 30 min, respectively
25 - 95
-
no or limited loss of activity at pH 7.0 and pH 10.0, respectively, 50% loss of activity at pH 3.0. Cooling from 95°C induces cold denaturation in partially denatured TIM
37
-
half-life of mutant enzyme H12N/K13G is 11.5 min
40
-
5 min, cytosolic enzyme, about 50% of activity
48
-
the melting temperature of the active form P1 of TIM is 48°C
51
-
half-life of mutant enzyme H13G is 11.5 min
55
-
5 min, chloroplastic enzyme, about 30% loss of activity
63
if denaturation is carried out at temperatures above 64.0°C, a partially folded species is formed in a time short enough to avoid the occurence of deleterious aggregation reactions
68
-
half-life of wild-type recombinant enzyme is 11.5 min
74.6
half-denaturation temperature
94
-
half-life of wild-type recombinant enzyme is 11.5 min
96
-
half-life of mutant enzyme H12N/K13G is 11.5 min
additional information