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5.3.1.1: triose-phosphate isomerase

This is an abbreviated version!
For detailed information about triose-phosphate isomerase, go to the full flat file.

Word Map on EC 5.3.1.1

Reaction

D-glyceraldehyde 3-phosphate
=
glycerone phosphate

Synonyms

CP 25, CTIMC, cTPI, cytoplasmic TPI, cytoplasmic triosephosphate isomerase, cytoTPI, D-glyceraldehyde-3-phosphate ketol-isomerase, GlTIM, Isomerase, triose phosphate, Lactacin B inducer protein, monoTIM, PfTIM, PfuTIM, Phosphotriose isomerase, plastidic TPI, plastidic triosephosphate isomerase, pTPI, SSO2592, TcTIM, TIM, TIM1, TIM2, TonTIM, TpI, TPI1, TpiA, Triose phosphate isomerase, Triose phosphate mutase, Triose phosphoisomerase, Triosephosphate isomerase, Triosephosphate mutase, vTIM

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.1 Interconverting aldoses and ketoses, and related compounds
                5.3.1.1 triose-phosphate isomerase

Purification

Purification on EC 5.3.1.1 - triose-phosphate isomerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3 distinct electrophoretic forms: I, II, III
-
3 isoenzymes
ammonium sulfate precipitation and CM-Sepharose column chromatography
-
ammonium sulfate precipitation and Mono-Q column column chromatography
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl-200 gel filtration
ammonium sulfate precipitation, Toyopearl column chromatography and SP-Sepharose column chromatography
CM Sepharose column chromatography
-
DEAE-Sepharose column chromatography
genetically engineered enzyme variant
-
HisTrap column chromatography Superdex 75 and gel filtration
native enzyme 2400fold from cell surface by hydrophobic interaction and anion exchange chromatography, followed by adsorption chromatography
-
native enzyme from shoots in a chromatographic process
-
Ni-NTA agarose column chromatography
-
Ni-NTA column chromatography
Ni-NTA column chromatography, Q-Sepharose column chromatography and Sephadex G-100 gel filtration
-
Ni-NTA His-Bind resin column chromatography, and Superdex 200 gel filtration
Ni2+ HiTrap chelating resin column chromatography
recombinant C-terminally His6-tagged TIM from Escherichia coli by metal affinity chromatography
recombinant enzyme
recombinant enzyme from Escherichia coli strain M15 to homogeneity by nickel affinity chromatography and gel filtration
-
recombinant monomeric enzyme from Escherichia coli strain BL21 (DE3)
recombinant mutant E104D
recombinant TIM from Escherichia coli
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange chromatography
-
recombinant wild-type and mutant enzymes from TIM-deficient Escherichia coli strain AA200 by gel filtration and ion-exchange chromatography
recombinant wild-type enzyme from TIM-deficient Escherichia coli strain DF502 by anion exchange chromatography
-
wild-type and mutant enzyme A238S
-
wild-type and mutant enzyme H12N/K13G
-
wild-type and mutant enzyme Y74C
-
wild-type and mutant enzymes H12N, H13G and H12N/K13G
-
wild-type enzyme and mutant enzymes C14A, C14P, C14S, C14T, C14V and C14G. Attempts to purify mutant enzymes C14M and C14L are unsuccessful, the enzymes exhibit a strong tendency to undergo aggregation
-
wild-type enzyme and mutant enzymes Y74C and Y74G