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5.3.1.1: triose-phosphate isomerase

This is an abbreviated version!
For detailed information about triose-phosphate isomerase, go to the full flat file.

Word Map on EC 5.3.1.1

Reaction

D-glyceraldehyde 3-phosphate
=
glycerone phosphate

Synonyms

CP 25, CTIMC, cTPI, cytoplasmic TPI, cytoplasmic triosephosphate isomerase, cytoTPI, D-glyceraldehyde-3-phosphate ketol-isomerase, GlTIM, Isomerase, triose phosphate, Lactacin B inducer protein, monoTIM, PfTIM, PfuTIM, Phosphotriose isomerase, plastidic TPI, plastidic triosephosphate isomerase, pTPI, SSO2592, TcTIM, TIM, TIM1, TIM2, TonTIM, TpI, TPI1, TpiA, Triose phosphate isomerase, Triose phosphate mutase, Triose phosphoisomerase, Triosephosphate isomerase, Triosephosphate mutase, vTIM

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.1 Interconverting aldoses and ketoses, and related compounds
                5.3.1.1 triose-phosphate isomerase

Cloned

Cloned on EC 5.3.1.1 - triose-phosphate isomerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
clone identified in screen for arsenate resistance
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of C-terminally His6-tagged TIM in Escherichia coli cytoplasm in a natively folded, enzymatically active form
expressed in Escherichia coli (DE3)pLysS cells
expressed in Escherichia coli AA200 cells
expressed in Escherichia coli AA200 cells (a null mutant of inherent TIM gene)
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21 pLys S cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) or BL21-CodonPlus(DE3)RIL cells. The preparations of native TIM contain two isolatable stable catalytically active forms P1 and P2 of TIM
-
expressed in Escherichia coli BL21(DE3)-R3-pRARE2 cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli FB215471(DE3) cells
-
expressen in Escherichia coli
-
expression as wild-type enzyme and fusion protein with an N-terminal tail of six histidine residues
-
expression in Escherichia coli
expression in Escherichia coli TOP10
-
expression in Saccharomyces cerevsiae
-
expression of the monomeric enzyme in Escherichia coli strain BL21 (DE3)
expression of the N-terminally His6-tagged enzyme in Escherichia coli strain M15
-
expression of the wild-type enzyme in TIM-deficient Escherichia coli strain DF502
-
expression of TIM in Escherichia coli
expression of TPI in Spodoptera frugiperda Sf21 cells using the baculovirus transfection system
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
expression of wild-type and mutant enzymes in TIM-deficient Escherichia coli strain AA200
expression of wild-type enzyme and mutant enzymes C126S and C126A in Escherichia coli
-
in expression vector pET-23(a) with C-terminal His6 tag fused in frame, expressed in Escherichia coli BL21(DE3) cells
mutant enzyme K12G is expressed in Escherichia coli strain DF502
-
mutant enzymes E97Q and E97D are expressed in Escherichia coli AA200 cells
overexpression in Escherichia coli
-
overexpression in Escherichia coli in fusion with glutathione S-transferase. The GST-PfuTIM fusion product partitions mainly into the insoluble fraction of the whole cell lysate
-
overexpression of the enzyme in Escherichia coli strain M15
-