5.2.1.4: maleylpyruvate isomerase

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For detailed information about maleylpyruvate isomerase, go to the full flat file.

Word Map on EC 5.2.1.4

5.2.1.4

gentisate

homeobox

fumarylpyruvate

1,2-dioxygenase

homeobox-containing

insertion-deletion

ciona

glutathione-independent

medicine

degradation

Reaction

Synonyms

3-maleylpyruvate cis-trans-isomerase, BagL, HbzG, Isomerase, maleylpyruvate, maleylpyruvate isomerase, MDMPI, MPI, msh gene, mycothiol-dependent maleylpyruvate isomerase, NagL, NarL, ncgl2918 gene, thiol-dependent maleylpyruvate isomerase

ECTree

5 Isomerases

5.2 cis-trans-Isomerases

5.2.1 cis-trans Isomerases (only sub-subclass identified to date)

5.2.1.4 maleylpyruvate isomerase

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Results	in table
19	Activating Compound
842	AA Sequence
3	Application
1	CAS Registry Number
9	Cloned(Commentary)
5	Cofactor
1	Crystallization (Commentary)
2	Expression
6	General Information
13	Inhibitors
9	kcat/KM [mM/s]
10	KM Value [mM]
4	Metals/Ions
8	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
62	Organism
7	Pathway
2	PDB ID
3	pH Optimum
2	pH Range
33	Protein Variants
8	Purification (Commentary)
2	Reaction
5	Reaction Type
46	Reference
7	Source Tissue
5	Specific Activity [micromol/min/mg]
2	Storage Stability
60	Substrates and Products (Substrate)
8	Subunits
26	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
9	Turnover Number [1/s]

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Cloned on EC 5.2.1.4 - maleylpyruvate isomerase

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expressed in Escherichia coli Rosetta (DE3) pLysS cells

Rhodococcus sp.

710986

expression in Escherichia coli

4 entries

gene bagL, DNA and amino acid sequence determination and analysis, phylogenetic analysis, comparison of genetic organization of gentisate catabolic gene clusters, recombinant expression of the N-terminall His6-tagged enzyme in Escherichia coli strain BL21(DE3)

Paenibacillus sp.

H6TG59

727760

N-terminal His-tagged wild type enzyme and mutants are expressed in Escherichia coli BL21(DE3) cells

Ralstonia sp.

714063

overexpression in Escherichia coli

Pseudomonas alcaligenes

746830

transformation into Escherichia coli 5K

Klebsiella pneumoniae

2488