5.2.1.1: maleate isomerase

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For detailed information about maleate isomerase, go to the full flat file.

Word Map on EC 5.2.1.1

5.2.1.1

fumarate

alcaligenes

faecalis

isomerization

serratia

racemase

stearothermophilus

marcescens

synthesis

Reaction

Synonyms

Isomerase, maleate, MaiA, maleate cis-trans isomerase, Pp-Iso

ECTree

5 Isomerases

5.2 cis-trans-Isomerases

5.2.1 cis-trans Isomerases (only sub-subclass identified to date)

5.2.1.1 maleate isomerase

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Results	in table
6	Activating Compound
2603	AA Sequence
1	Application
1	CAS Registry Number
4	Cloned(Commentary)
3	Crystallization (Commentary)
4	General Information
1	General Stability
36	Inhibitors
5	kcat/KM [mM/s]
1	Ki Value [mM]
9	KM Value [mM]
3	Metals/Ions
9	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
27	Organism
1	Oxidation Stability
11	Pathway
8	PDB ID
9	pH Optimum
8	pH Range
4	pH Stability
24	Protein Variants
6	Purification (Commentary)
1	Reaction
3	Reaction Type
23	Reference
4	Specific Activity [micromol/min/mg]
2	Storage Stability
33	Substrates and Products (Substrate)
8	Subunits
8	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
5	Temperature Range [°C]
13	Temperature Stability [°C]
7	Turnover Number [1/s]

Purification

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Purification on EC 5.2.1.1 - maleate isomerase

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3 entries

recombinant mutant MaiR-D48/49-304AA 2.7fold from Escherichia coli strain Rosetta 2 (DE3) by ammonium sulfate fractionation, anion exchange chromatography, ultratfiltration, and gel filtration

Rhodococcus jostii

Q0SJH2

728167

recombinant N- and C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration

Pseudomonas putida

F8G0M3

728334