5.1.99.7: dihydroneopterin triphosphate 2'-epimerase
This is an abbreviated version!
For detailed information about dihydroneopterin triphosphate 2'-epimerase, go to the full flat file.
Word Map on EC 5.1.99.7
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5.1.99.7
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epimerization
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antiparallel
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6-hydroxymethyl-7,8-dihydropterin
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cyclohydrolase
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purine
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folate
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tetrahydrofolate
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homo-octamer
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pteridine
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drug development
- 5.1.99.7
-
epimerization
-
antiparallel
- 6-hydroxymethyl-7,8-dihydropterin
-
cyclohydrolase
- purine
- folate
- tetrahydrofolate
-
homo-octamer
- pteridine
- drug development
Reaction
Synonyms
D-erythro-7,8-dihydroneopterin triphosphate epimerase, dihydroneopterin triphosphate epimerase, dihydroneopterin-triphosphate epimerase, folX, H2NTP 2'-epimerase
ECTree
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Purification
Purification on EC 5.1.99.7 - dihydroneopterin triphosphate 2'-epimerase
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native enzyme 954fold by ammonium sulfate fractionation, Cibacron blue 3GA affinity chromatography, hydrophobic interaction chromatography, methotrexate affinity chromatography, and ge filtration, to homogeneity
recombinant enzyme 2.7fold from Escherichia coli strain M15 by anion exchange chromatography, ultrafiltration, heat treatment at 80°C for 4 min, and gel filtration
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