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5.1.99.7: dihydroneopterin triphosphate 2'-epimerase

This is an abbreviated version!
For detailed information about dihydroneopterin triphosphate 2'-epimerase, go to the full flat file.

Word Map on EC 5.1.99.7

Reaction

7,8-dihydroneopterin 3'-triphosphate
=
7,8-dihydromonapterin 3'-triphosphate

Synonyms

D-erythro-7,8-dihydroneopterin triphosphate epimerase, dihydroneopterin triphosphate epimerase, dihydroneopterin-triphosphate epimerase, folX, H2NTP 2'-epimerase

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.99 Acting on other compounds
                5.1.99.7 dihydroneopterin triphosphate 2'-epimerase

Purification

Purification on EC 5.1.99.7 - dihydroneopterin triphosphate 2'-epimerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 954fold by ammonium sulfate fractionation, Cibacron blue 3GA affinity chromatography, hydrophobic interaction chromatography, methotrexate affinity chromatography, and ge filtration, to homogeneity
recombinant enzyme
recombinant enzyme 2.7fold from Escherichia coli strain M15 by anion exchange chromatography, ultrafiltration, heat treatment at 80°C for 4 min, and gel filtration
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