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5.1.3.37: mannuronan 5-epimerase

This is an abbreviated version!
For detailed information about mannuronan 5-epimerase, go to the full flat file.

Word Map on EC 5.1.3.37

Reaction

[mannuronan]-beta-D-mannuronate
=
[alginate]-alpha-L-guluronate

Synonyms

AlgE1, AlgE2, AlgE3, AlgE4, AlgE5, AlgE6, AlgE7, algG, alginate epimerase, C5-mannuronan epimerase, c5epi, ManC5-E, mannuronan C5-epimerase, MC5E, MEP13, MEP13-C5, MEP18, MEP18-C5, Mep2, MEP2-C5, MEP21, MEP21-C5, MEP25, MEP25-C5, MEP26, MEP26-C5, MEP27, MEP27-C5, MEP28, MEP28-C5, MEP29, MEP29-C5, Mep4, MEP4-C5, Mep6, MEP6-C5, Mep7, MEP7-C5, poly(beta-D-mannuronate) C5 epimerase 4

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.37 mannuronan 5-epimerase

Purification

Purification on EC 5.1.3.37 - mannuronan 5-epimerase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
rapid purification procedure based on fast protein liquid chromatography on MonoQ resin
-
recombinant enzyme AlgE4 and chimeric enzyme mutant AlgE64 from Escherichia coli strain ER2566 by chitin affinity chromatography, dialysis, and gel filtration
recombinant enzyme AlgE6 and chimeric enzyme mutant AlgE64, and of recombinant R-modules from Escherichia coli strain ER2566 by chitin affinity chromatography, dialysis, and gel filtration
recombinant His-tagged catalytic domain, MEP13-C5, solubilized from Escherichia coli strain BL21 CodonPlus(DE3)RIPL inclusion bodies
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by gel filtration, nickel affinity and chitin affinity chromatography
recombinant secreted His-tagged MC5E clone SjC5-VI from Spodoptera frugiperda Sf9 cells by dialysis and nickel affinity chromatography
using a Sepharose 6B column containing alginate covalently linked to the hydroxyl groups
-