5.1.3.3: Aldose 1-epimerase

This is an abbreviated version!
For detailed information about Aldose 1-epimerase, go to the full flat file.

Word Map on EC 5.1.3.3

5.1.3.3

anomer

mutarotation

leloir

beta-anomers

alpha-d-galactose

beta-d-galactose

galactokinase

alpha-d-glucose

gale

analysis

synthesis

Reaction

Synonyms

aldose 1-epimerase, aldose 1-epimerase-like protein, aldose mutarotase, aldose-1-epimerase, aldose-l-epimerase, Epimerase, aldose-1, Gal10p, galactomutarotase, galactose mutarotase, galactose mutarotase/UDP-galactose 4-epimerase protein, GalM, Mutarotase, mutarotase YeaD, NbAELP, stYeaD, YeaD

ECTree

5 Isomerases

5.1 Racemases and epimerases

5.1.3 Acting on carbohydrates and derivatives

5.1.3.3 Aldose 1-epimerase

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Results	in table
23636	AA Sequence
4	Application
2	CAS Registry Number
19	Cloned(Commentary)
1	Cofactor
10	Crystallization (Commentary)
9	Disease/ Diagnostics
1	Expression
10	General Information
2	General Stability
87	Inhibitors
84	KM Value [mM]
8	Localization
1	Metals/Ions
44	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
78	Organism
9	Pathway
59	PDB ID
9	pH Optimum
2	pH Range
6	pH Stability
3	pI Value
14	Protein Variants
17	Purification (Commentary)
9	Reaction
2	Reaction Type
73	Reference
36	Source Tissue
16	Specific Activity [micromol/min/mg]
1	Storage Stability
126	Substrates and Products (Substrate)
23	Subunits
28	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Range [°C]
9	Temperature Stability [°C]
33	Turnover Number [1/s]

Purification

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Purification on EC 5.1.3.3 - Aldose 1-epimerase

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7 entries

enzyme form I and II from kidney and enzyme form II from liver

Rattus norvegicus

2376

multiple forms: I, II, III, and IV. Two major forms: I and II are purified

Sus scrofa

2375

native enzyme 8.35fold from liver by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration

Bos taurus

728838

Ni-NTA affinity column chromatography and Superdex 200 gel filtration

Escherichia coli

713653

NiNTA affinity chromatography

Salmonella enterica subsp. enterica serovar Typhimurium

671057

NiNTA affinity column chromatography

Salmonella enterica

671057

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Salmonella enterica subsp. enterica serovar Typhimurium

671057

recombinant His6-tagged enzyme from Escherichia coli strain MG1655

Yersinia pestis

728233

UDPgalactose 4-epimerase is a bifunctional enzyme with aldose 1-epimerase activity. The active sites for the two enzymatic activities are located in different regions of the epimerase homoenzyme

Saccharomyces cerevisiae

651176