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5.1.3.3: Aldose 1-epimerase

This is an abbreviated version!
For detailed information about Aldose 1-epimerase, go to the full flat file.

Word Map on EC 5.1.3.3

Reaction

alpha-D-glucose
=
beta-D-glucose

Synonyms

aldose 1-epimerase, aldose 1-epimerase-like protein, aldose mutarotase, aldose-1-epimerase, aldose-l-epimerase, Epimerase, aldose-1, Gal10p, galactomutarotase, galactose mutarotase, galactose mutarotase/UDP-galactose 4-epimerase protein, GalM, Mutarotase, mutarotase YeaD, NbAELP, stYeaD, YeaD

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.3 Aldose 1-epimerase

Crystallization

Crystallization on EC 5.1.3.3 - Aldose 1-epimerase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 200 mM sodium acetate and 100 mM Tris-HCl pH 8.5
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both apoform and in complex with beta-D-galactose, comparison with enzyme from Lactococcus lactis and Caenorhabditis elegans
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both apo-form and in complex with alpha,beta-D-galactose
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in complex with D-glucose, D-fucose, D-quinovose, L-arabinose, or D-xylose
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mutants H96N, H170N, D243N, D243A, E304Q, E304A, complexed with either D-glucose or D-galactose
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in complex with NAD+, UDP-glucose, and beta-D-galactose
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hanging-drop vapour-diffusion method
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purified recombinant His-tagged enzyme, hanging-drop vapour-diffusion method, 0.003 ml of 10 mg/ml protein in 15-25% PEG 8000, 0.1 M Na citrate, pH 5.5, and 0.2-0.5 mM NaCl for monoclinic crystals, or in 1.5-2 M ammonium sulfate, 25 mM Mg formate, 0.1 M Na cacodylate, pH 5.5, for orthorhombic crystals, cocrystallization of YeaD with ligands glucose, galactose, mannose, fructose, fucose, xylose, lyxose, lactose, maltose, sucrose and glucose 6-phosphate, incubation at 4°C for 12 h in a 1:10 molar ratio, X-ray diffraction structure determination and analysis the orthorhombic form at 1.9 A resolution and in the monoclinic form at 2.5 A resolution
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