5.1.3.20: ADP-glyceromanno-heptose 6-epimerase

This is an abbreviated version!
For detailed information about ADP-glyceromanno-heptose 6-epimerase, go to the full flat file.

Reaction

Synonyms

Adenosine diphosphoglyceromannoheptose 6-epimerase, ADP-glycero-manno-heptose-6-epimerase, ADP-glyceromanno-heptose 6-epimerase, ADP-L,D-hep 6-epimerase, ADP-L-glycero-D-manno-heptose 6-epimerase, ADP-L-glycero-D-manno-heptose-6-epimerase, ADP-L-glycero-D-manno-heptose-epimerase, Aquifex aelicis gene rfaD, ADP-L-glycero-D-mannoheptose 6-epimerase, ADP-L-glycero-D-mannoheptose 6-epimerase (Neisseria gonorrhoeae gene gme), ADP-L-glycero-D-mannoheptose-6-epimerase, ADP-L-glycero-D-mannoheptose-6-epimerase (Escherichia coli strain K-12 substrain MG1655 clone EC19-98 gene rfaD), AGME, Epimerase, adenosine diphosphoglyceromannoheptose 6-, Epimerase, adenosine diphosphoglyceromannoheptose 6- (Escherichia coli clone pCG50 reduced), Epimerase, adenosine diphosphoglyceromannoheptose 6- (Neisseria gonorrhoeae gene lis-6), Genbank AE000440-derived protein GI 1790049, GenBank AE000684-derived protein GI 2983012, GmhD, hldD, HP0859, RfaD

ECTree

     5 Isomerases

         5.1 Racemases and epimerases

             5.1.3 Acting on carbohydrates and derivatives

                5.1.3.20 ADP-glyceromanno-heptose 6-epimerase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
8796	AA Sequence
4	CAS Registry Number
12	Cloned(Commentary)
13	Cofactor
5	Crystallization (Commentary)
10	General Information
5	Inhibitors
2	KM Value [mM]
4	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
24	Organism
3	Pathway
55	PDB ID
1	pH Optimum
1	pI Value
1	Posttranslational Modification
11	Protein Variants
6	Purification (Commentary)
3	Reaction
2	Reaction Type
23	Reference
2	Specific Activity [micromol/min/mg]
24	Substrates and Products (Substrate)
11	Subunits
45	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]

Crystallization

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Crystallization on EC 5.1.3.20 - ADP-glyceromanno-heptose 6-epimerase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Crystallization (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, mixing of 200 nl of 8.8 mg/ml protein in 0.43 M NaCl, 20 mM Tris-HCl, pH 8.0, with 200 nl of reservoir solution containing 35% PEG 200, 0.1 M bis-tris-HCl, pH 5.5, and equilibration against 0.05 ml of reservori solution, method optimization, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement

Burkholderia thailandensis

Q2SY18

726590

hanging-drop vapor-diffusion method, three crystal forms

Escherichia coli

-

649112

structure of the enzyme in complex with NADP+ and the inhibitor ADP-glucose, determined at 2.0 A resolution by multiwavelength anomalous diffraction phasing method

Escherichia coli

-

653940

Y140F mutant enzyme bound to ADP-beta-D-mannose, hanging drop vapor diffusion method, using 2M (NH4)2SO4, 0.1 M HEPES-Na, pH 7.1, 2% (w7v) PEG 400

Escherichia coli

P67910

716848

purified recombinant His-tagged enzyme, vapor diffusion technique, mixing of 20 mg/ml protein in 30 mM Tris, pH 7.5, 150 mM NaCl, with precipitant solution containing 0.2 M ammonium sulfate, 0.1 M tri sodium citrate, pH 5.6, 15% w/v PEG 4000, and 5% glycerol, 20°C, X-ray diffraction structure determination and analysis at 2.55 A resolution

Helicobacter pylori

B5Z7L9

727070