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5.1.3.19: chondroitin-glucuronate 5-epimerase

This is an abbreviated version!
For detailed information about chondroitin-glucuronate 5-epimerase, go to the full flat file.

Word Map on EC 5.1.3.19

Reaction

chondroitin D-glucuronate
=
dermatan L-iduronate

Synonyms

Chondroitin D-glucuronosyl 5-epimerase, chondroitin-glucuronate C5-epimerase, dermatan sulfate epimerase, dermatan sulfate epimerase 1, dermatan sulfate epimerase 2, dermatan sulfate epimerase like, dermatan sulfate epimerase-2, dermatan sulfate epimerase-like a, dermatan sulfate epimerase-like b, Dermatan-sulfate 5-epimerase, DS epimerase 1, DS epimerase 2, DS-epi1, DS-epi2, DS-epimerase 1, DSE, DSEL, dsela, dselb, Epimerase, chondroitin glucuronate 5-, Polyglucuronate 5-epimerase, SART2

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.19 chondroitin-glucuronate 5-epimerase

Engineering

Engineering on EC 5.1.3.19 - chondroitin-glucuronate 5-epimerase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H205A
site-directed mutagenesis of the catalytic site residue, the mutant shows complete loss of epimerase activity
H450A
site-directed mutagenesis of the catalytic site residue, the mutant shows complete loss of epimerase activity
N183S
site-directed mutagenesis of an N-glycosylation site in DS-epi1 altering reaction kinetics
N336S
site-directed mutagenesis of an N-glycosylation site in DS-epi1 altering reaction kinetics and reducing the activity 13fold
N642S
site-directed mutagenesis of an N-glycosylation site in DS-epi1 altering reaction kinetics
N648S
site-directed mutagenesis of an N-glycosylation site in DS-epi1 altering reaction kinetics and reducing the activity 43fold
S268L
a naturally occuring homozygous DSE mutation c.803C>T in a patient with musculocontractural type of Ehlers-Danlos syndrome, MCEDS. The mutant enzymes shows a loss of activity towards partially desulfated dermatan sulfate, patient-derived fibroblasts also show a significant reduction in epimerase activity
Y261A
site-directed mutagenesis of the catalytic site residue, the mutant shows complete loss of epimerase activity
additional information
-
siRNA gene silencing of DS-epi2 and DS-epi1 in lung HFL-1 fibroblasts