5.1.3.14: UDP-N-acetylglucosamine 2-epimerase (non-hydrolysing)

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For detailed information about UDP-N-acetylglucosamine 2-epimerase (non-hydrolysing), go to the full flat file.

Reaction

Synonyms

bas5048, bas5117, Bs-epimerase, Cap5P, Epimerase, uridine diphosphoacetylglucosamine 2-, GNE, GNE/MNK, GNE1, GNE2, GneY, GneZ, His-rGNE, Mj-epimerase, MnaA, More, NeuC protein, NmSacA, non-hydrolyzing Neisseria meningitidis serogroup A UDP-GlcNAc 2-epimerase, non-hydrolyzing Neisseria meningitidis serogroup A UDP-N-acetylglucosamine 2-epimerase, non-hydrolyzing UDP-GlcNAc 2-epimerase, non-hydrolyzing UDP-N-acetylglucosamine 2-epimerase, non-hydrolyzing uridine 5'-diphosphate-N-acetylglucosamine 2-epimerase, SacA, teichoic acid 2-epimerase, UDP-GlcNAc 2'-epimerase, UDP-GlcNAc 2-epimerase, UDP-GlcNAc 2-epimerase/ManAc kinase, UDP-GlcNAc 2-epimerase/ManNAc kinase, UDP-GlcNAc 2-epimerase/ManNAc kinase gene, UDP-GlcNAc-2-epimerase, UDP-GlcNAc:UDP-ManNAc 2-epimerase, UDP-N-acetylglucosamine 2'-epimerase, UDP-N-acetylglucosamine 2-epimerase, UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, UDP-N-acetylglucosamine-2-epimerase, UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase, uridine diphosphate N-acetylglucosamine 2-epimerase, Uridine diphosphate-N-acetylglucosamine-2'-epimerase, Uridine diphospho-N-acetylglucosamine 2'-epimerase, Uridine diphosphoacetylglucosamine 2'-epimerase, wecB, WTA 2-epimerase

ECTree

     5 Isomerases

         5.1 Racemases and epimerases

             5.1.3 Acting on carbohydrates and derivatives

                5.1.3.14 UDP-N-acetylglucosamine 2-epimerase (non-hydrolysing)

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Results	in table
11	Activating Compound
14738	AA Sequence
9	Application
1	CAS Registry Number
29	Cloned(Commentary)
7	Crystallization (Commentary)
219	Disease/ Diagnostics
46	General Information
3	General Stability
123	Inhibitors
2	kcat/KM [mM/s]
3	Ki Value [mM]
26	KM Value [mM]
28	Localization
4	Metals/Ions
16	Molecular Weight [Da]
51	Natural Substrates/ Products (Substrates)
101	Organism
13	Pathway
41	PDB ID
13	pH Optimum
4	pH Range
3	Posttranslational Modification
121	Protein Variants
21	Purification (Commentary)
13	Reaction
1	Reaction Type
91	Reference
59	Source Tissue
8	Specific Activity [micromol/min/mg]
10	Storage Stability
123	Substrates and Products (Substrate)
27	Subunits
128	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
12	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 5.1.3.14 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolysing)

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-10°C, overnight, in a saturated (NH4)2SO4 solution, approximately 20% loss of activity

Rattus norvegicus

-

2259

-18�C, stable for several months

2 entries

-70°C, stable for several months

Rattus norvegicus

-

2280

2°C, several days, maintains well over 50% of its activity

Rattus norvegicus

-

2257

4°C, 80-90% loss of activity after 60 h

Rattus norvegicus

-

2258

4°C, in presence of 0.1 mM UDP and 1 mM DTT, 3 days, 20% loss of activity

Rattus norvegicus

-

2261

4°C, purified enzyme expressed in insect cells, stable

Rattus norvegicus

-

663299

4°C, purified enzyme is 80% pure, no further purification because it is unstable to freezing and has limited stability upon storage at 4°C

Rattus norvegicus

-

651592

-18�C, stable for several months

Escherichia coli

-

2265, 2268

-18�C, stable for several months

Bacillus cereus

-

2264