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5.1.3.14: UDP-N-acetylglucosamine 2-epimerase (non-hydrolysing)

This is an abbreviated version!
For detailed information about UDP-N-acetylglucosamine 2-epimerase (non-hydrolysing), go to the full flat file.

Word Map on EC 5.1.3.14

Reaction

UDP-N-acetyl-alpha-D-glucosamine
=
UDP-N-acetyl-alpha-D-mannosamine

Synonyms

bas5048, bas5117, Bs-epimerase, Cap5P, Epimerase, uridine diphosphoacetylglucosamine 2-, GNE, GNE/MNK, GNE1, GNE2, GneY, GneZ, His-rGNE, Mj-epimerase, MnaA, More, NeuC protein, NmSacA, non-hydrolyzing Neisseria meningitidis serogroup A UDP-GlcNAc 2-epimerase, non-hydrolyzing Neisseria meningitidis serogroup A UDP-N-acetylglucosamine 2-epimerase, non-hydrolyzing UDP-GlcNAc 2-epimerase, non-hydrolyzing UDP-N-acetylglucosamine 2-epimerase, non-hydrolyzing uridine 5'-diphosphate-N-acetylglucosamine 2-epimerase, SacA, teichoic acid 2-epimerase, UDP-GlcNAc 2'-epimerase, UDP-GlcNAc 2-epimerase, UDP-GlcNAc 2-epimerase/ManAc kinase, UDP-GlcNAc 2-epimerase/ManNAc kinase, UDP-GlcNAc 2-epimerase/ManNAc kinase gene, UDP-GlcNAc-2-epimerase, UDP-GlcNAc:UDP-ManNAc 2-epimerase, UDP-N-acetylglucosamine 2'-epimerase, UDP-N-acetylglucosamine 2-epimerase, UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, UDP-N-acetylglucosamine-2-epimerase, UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase, uridine diphosphate N-acetylglucosamine 2-epimerase, Uridine diphosphate-N-acetylglucosamine-2'-epimerase, Uridine diphospho-N-acetylglucosamine 2'-epimerase, Uridine diphosphoacetylglucosamine 2'-epimerase, wecB, WTA 2-epimerase

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.14 UDP-N-acetylglucosamine 2-epimerase (non-hydrolysing)

Cloned

Cloned on EC 5.1.3.14 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolysing)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
bifunctional enzyme EC 5.1.3.14/EC 2.7.1.60, expressed in COS7 cells
cloning of the neuC gene into an intein expression vector to facilitate purification
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DNA sequencing of the GNE coding region of 64 symptomatic patients with autosomal recessive hereditary inclusion body myopathy, genotyping-phenotyping of patients from different ethnics, overview
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expression in Arabidopsis thaliana
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expression in Chinese Hamster Ovary (CHO)
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expression in Escherichia coli
expression in Escherichia coli, C-terminal His-tag
expression in insect cells
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expression in Insect Cells and supercompetent InvalphaF' Escherichia coli cells (Invitrogen)
expression in Sf9 insect cells, wild-type enzyme and mutant enzymes DELTA1-39, DELTA1-234, DELTA1-356, DELTA383-722, DELTA490-722, DELTA597-722, DELTA697-722, DELTA717-722
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expression of M712T mutant enzyme in Sf9 insect cells
expression of wild-type and mutant enzymes (C13S, H132Q, D176V, D177C, V331A, I472T, G708S, A631V, V572L and A630T) in COS-7 cells
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functional expression in Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris or insect cells. In all cell types, the expressed enzyme displayes both epimerase and kinase activities. In Escherichia coli up to 2 mg protein/l cell culture is expressed in yeast cells only 0.4 mg/ml, in insect cells up to 100 mg/L. In all three cell systems, insoluble protein aggregates are also observed. Expression and purification of the UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase in Sf-900 cells can yield the miligram amount of protein required for structural characterization of the enzyme. The easier expression in Escherichia coli and yeast provides sufficient quantities for enzymatic and kinetic characterization
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gene gneY, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons
Q81K32, Q81X16
gene gneYZ, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons
Q81K32, Q81X16
gene mnaA, genotyping and mutation identification
gene sacA, encoded in the serogroup A cps operon, sequence comparisons, recombinant overexpression of C-terminally His6-tagged recombinant NmSacA in Escherichia coli strain BL21(DE3)
Neisseria meningitidis serogroup A / serotype 4A
gene sacA, recombinant expression of C-terminally His6-tagged enzyme NmSacA in Escherichia coli strain BL21(DE3)
Neisseria meningitidis serogroup A /serotype 4A
gene wecB, sequence comparisons, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene wecB, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
high level overexpression of the active enzyme is established by using the baculovirus/Sf9 system
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overexpression in Escherichia coli
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overexpression in Escherichia coli BL21(DE3), wild-type enzyme and mutant enzymes D100N, E122Q and D131N
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proteome expression analysis in wild-type and GNE-deficient embryonic stem cells, overview. GNE overexpression in HEK cells
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splice variant hGNE2, expression in insect and mammalian cells. Splice variant hGNE3, expression in Escherichia coli
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splice variants mGNE1, mGNE2, mGNE3
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wild-type and mutant enzymes expressed in Escherichia coli
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