5.1.3.13: dTDP-4-dehydrorhamnose 3,5-epimerase

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For detailed information about dTDP-4-dehydrorhamnose 3,5-epimerase, go to the full flat file.

Reaction

Synonyms

3,5-epimerase, Agl13, bifunctional UDP-4-keto-6-deoxy-D-glucose epimerase/reductase, C14F11.6, deoxythymidine diphosphate-4-dehydrorhamnose 3,5-epimerase, deoxythymidine diphospho-4-keto-6-deoxyglucose 3,5-epimerase, DnmU, dTDP-4-dehydro-6-deoxy-glucose-3,5-epimerase, dTDP-4-dehydrorhamnose 3,5-epimerase, dTDP-4-keto-6-deoxy-D-hexulose 3,5-epimerase, dTDP-4-keto-6-deoxyglucose 3,5-epimerase, dTDP-4-keto-L-rhamnose 3,5-epimerase, dTDP-6-deoxy-D-xylo-4-hexulose 3',5'-epimerase, dTDP-L-rhamnose synthetase, Epimerase, thymidine diphospho-4-ketorhamnose 3,5-, NovW gene product, RfbC, RmbC, rml-3, RmlC, TDP-4-keto-6-deoxy-D-glucose 3,5-epimerase, TDP-4-keto-L-rhamnose-3,5-epimerase, TDP-4-ketorhamnose 3,5-epimerase, TDP-6-deoxy-D-xylo-4-hexopyranosid-4-ulose 3,5-epimerase, Thymidine diphospho-4-ketorhamnose 3,5-epimerase, UGER

ECTree

     5 Isomerases

         5.1 Racemases and epimerases

             5.1.3 Acting on carbohydrates and derivatives

                5.1.3.13 dTDP-4-dehydrorhamnose 3,5-epimerase

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Results	in table
29027	AA Sequence
6	Application
1	CAS Registry Number
12	Cloned(Commentary)
5	Cofactor
6	Crystallization (Commentary)
27	General Information
9	IC50 Value
10	Inhibitors
7	KM Value [mM]
1	Localization
2	Metals/Ions
8	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
221	Organism
11	Pathway
90	PDB ID
5	pH Optimum
6	Protein Variants
8	Purification (Commentary)
3	Reaction
5	Reaction Type
29	Reference
6	Source Tissue
32	Substrates and Products (Substrate)
6	Subunits
55	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Stability [°C]
6	Turnover Number [1/s]

Crystallization

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Crystallization on EC 5.1.3.13 - dTDP-4-dehydrorhamnose 3,5-epimerase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified mixture containing all four dTDP-L-rhamnose biosynthetic enzymes, RfbA, RfbB, RfbC and RfbD, sitting drop vapor diffusion method, mixing of 0.001 ml of protein solution, containing 10mM Tris-HCl, pH 8.3, 500 mM NaCl, 5 mM 2-mercaptoethanol, 1 mM dTTP, 1 mM Glc1P, 1 mM NAD+, 1 mM NADP+, and 1mM MgCl2, with 0.001 ml of reservoir solution containing 0.2 M diammonium citrate, pH 5.0, 20% w/v, and PEG 3350, at room temperature, X-ray diffraction structure determination and analysis at 1.63 A resolution, molecular replacement using one monomer from PDB ID 1dzr as the search model

Bacillus anthracis

Q81TP1

746670

hanging drop vapor diffusion method, crystal structure in presence and absence of dTDP

Methanothermobacter thermautotrophicus

-

652025

enzymatically

Mycobacterium tuberculosis

-

675420

incubating RmlC with 20 mM dTDP-6-deoxy-D-xylo-4-hexulose, for 2 h at room temperature, prior to setting up crystal plates with 25% PEG 8000, 0.2 M sodium tartrate, 0.1 M Mes (pH 6.2)

Pseudomonas aeruginosa

-

675420

the structure is determined by multiwavelength anomalous diffraction to a resolution of 2.17 A

Salmonella enterica

-

653311

sitting drop method, crystals of native and selenomethionine protein, crystals in complex with dTDP-D-glucose and dTDP-D-xylose

Streptococcus suis

-

653912