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5.1.3.13: dTDP-4-dehydrorhamnose 3,5-epimerase

This is an abbreviated version!
For detailed information about dTDP-4-dehydrorhamnose 3,5-epimerase, go to the full flat file.

Word Map on EC 5.1.3.13

Reaction

dTDP-4-dehydro-6-deoxy-alpha-D-glucose
=
dTDP-4-dehydro-beta-L-rhamnose

Synonyms

3,5-epimerase, Agl13, bifunctional UDP-4-keto-6-deoxy-D-glucose epimerase/reductase, C14F11.6, deoxythymidine diphosphate-4-dehydrorhamnose 3,5-epimerase, deoxythymidine diphospho-4-keto-6-deoxyglucose 3,5-epimerase, DnmU, dTDP-4-dehydro-6-deoxy-glucose-3,5-epimerase, dTDP-4-dehydrorhamnose 3,5-epimerase, dTDP-4-keto-6-deoxy-D-hexulose 3,5-epimerase, dTDP-4-keto-6-deoxyglucose 3,5-epimerase, dTDP-4-keto-L-rhamnose 3,5-epimerase, dTDP-6-deoxy-D-xylo-4-hexulose 3',5'-epimerase, dTDP-L-rhamnose synthetase, Epimerase, thymidine diphospho-4-ketorhamnose 3,5-, NovW gene product, RfbC, RmbC, rml-3, RmlC, TDP-4-keto-6-deoxy-D-glucose 3,5-epimerase, TDP-4-keto-L-rhamnose-3,5-epimerase, TDP-4-ketorhamnose 3,5-epimerase, TDP-6-deoxy-D-xylo-4-hexopyranosid-4-ulose 3,5-epimerase, Thymidine diphospho-4-ketorhamnose 3,5-epimerase, UGER

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.13 dTDP-4-dehydrorhamnose 3,5-epimerase

Cloned

Cloned on EC 5.1.3.13 - dTDP-4-dehydrorhamnose 3,5-epimerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into pET23a(+) with an N-terminal 6× His tag and a linker consisting of Gly-Ser-Met-Ala, overexpression in Escherichia coli
-
cloned into pET23b using the Ligation Independent Clone system
-
expression in Escherichia coli
gene agl13, organized in a rmlABCD type cluster, sequence comparisons, functional recombinant expression of cellulose-bound CBD-Agl13 in Haloferax volcanii strain WR536
gene dnmU, DNA and amino acid sequence determination, analysis, and comparison, genetic organization, overview. Recombinant expression in Escherichia coli
gene L780, transcription profiling of UGER, phylogenetic analysis
E3VXL5
gene rmbC, DNA and amino acid sequence determination, analysis, and comparison, genetic organization, overview. Recombinant expression in Escherichia coli
gene rml-3, sequence comparisons and phyylogenetic analysis of rml genes, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with genes rml-1, rml-2, and rml-4
recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3) Magic
Q81TP1