5.1.3.11: cellobiose epimerase
This is an abbreviated version!
For detailed information about cellobiose epimerase, go to the full flat file.
Word Map on EC 5.1.3.11
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5.1.3.11
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synthesis
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epimerization
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lactulose
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caldicellulosiruptor
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saccharolyticus
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isomerization
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d-glucose
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ruminococcus
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albus
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prebiotic
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d-mannose
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marinus
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turgidum
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rhodothermus
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nutrition
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dictyoglomus
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mannans
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food industry
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n-acyl-d-glucosamine
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epimerizes
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isomerizes
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cello-oligosaccharides
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spirochaeta
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pharmacology
- 5.1.3.11
- synthesis
-
epimerization
- lactulose
- caldicellulosiruptor
- saccharolyticus
-
isomerization
- d-glucose
- ruminococcus
- albus
-
prebiotic
- d-mannose
- marinus
- turgidum
-
rhodothermus
- nutrition
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dictyoglomus
- mannans
- food industry
- n-acyl-d-glucosamine
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epimerizes
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isomerizes
- cello-oligosaccharides
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spirochaeta
- pharmacology
Reaction
Synonyms
B15CE, BfCE, BN699_02313, Caob-CE, CE-NE1, ce13, cellobiose 2-epimerase, CS-HRCE, Csac_0294, CsCE, CvCE, DfCE, Dfer_1975, DsCE, DtCE, Dtur_0652, EcCE, EpiA, epilactose-producing cellobiose 2-epimerase, Epimerase, cellobiose, FbCE, FjCE, Fjoh_4956, HaCE, Haur_0887, mannobiose 2-epimerase, md1, md2, PhCE, Phep_3983, RACE, Rmar_2439, RmCE, SdCE, Sde_0508, SlCE, Slin_0271, Spith_0082, StCE, STHERM_c00950, TERTU_4095, Thsa-CE, TtCE
ECTree
5.1.3.11 cellobiose epimeraseAdvanced search results
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results (Temperature Stability
Temperature Stability on EC 5.1.3.11 - cellobiose epimerase
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
30
40
50
60
60 - 80
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the half-lives of the enzyme at 60°C, 65°C, 70°C, 75°C, and 80°C are 142, 71, 35, 18, and 4.6 h, respectively
65
65 - 85
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purified recombinant His-tagged enzyme, thermal inactivation of the cellobiose 2-epimerase follows first-order kinetics and the half-lives of the enzyme at 65°C, 70°C, 75°C, 80°C, and 85°C are 79 h, 55 h, 35 h, 20 h, and 4.8 h, respectively
70
80
86.7
the enzyme displays high thermostability with Tm of 86.7 ?C
87.5
the most thermostable mutant E161D/N365P displays an approximate 20% increase in activity at 87.5°C compared with that at 80°C
95
recombinant wild-type enzyme loses more than 80% activity at 95°C, while mutants E161D/S180P/S351G and E161D/N365P retain more than 50% and 70% activity, respectively
additional information
40
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EpiA retains over 90% of its activity at pH values in the range of 5.2-10.0 and temperatures below 40°C following the pH (at 4°C for 24 h) and heat (at pH 8.0 for 30 min) treatment processes, respectively
80
purified recombinant enzyme, pH 7.0, 40 min, 80% of original activity is retained below 80°C
80
purified recombinant enzymes, wild-type enzyme CsCE has a half-life of thermal inactivation (t1/2) of approximately 140 min, while the half-lives of mutants E161D/S180P/S351G and E161D/N365P increase to 462.7 min and 554.0 min, respectively
80
t1/2: 141 min (wild-type enzym), 161.2 min (mutant enzyme A12S), 132.5 (mutant enzyme A12S/K328I), 116.9 min (mutant enzyme R5M/A12S/F231L/K328I), 159.4 min (mutant enzyme R5M/A12S/I52V/F231L/K328I)
80
t1/2: 141.3 min (wild-type enzyme), 462.7 min (mutant enzyme E161D/S180P/S351G), 554.0 min (mutant enzyme E161D/N365P)
additional information
the enzyme displays first-order kinetics for thermal inactivation, and the half-lives (inactivation rate constants) at 65°C, 70°C, 75°C, and 80°C are 74 h, 34 h, 16h , and 5 h, respectively
additional information
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the enzyme displays first-order kinetics for thermal inactivation, and the half-lives (inactivation rate constants) at 65°C, 70°C, 75°C, and 80°C are 74 h, 34 h, 16h , and 5 h, respectively
