5.1.3.11: cellobiose epimerase
This is an abbreviated version!
For detailed information about cellobiose epimerase, go to the full flat file.
Word Map on EC 5.1.3.11
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5.1.3.11
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synthesis
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epimerization
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lactulose
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caldicellulosiruptor
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saccharolyticus
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isomerization
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d-glucose
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ruminococcus
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albus
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prebiotic
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d-mannose
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marinus
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turgidum
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rhodothermus
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nutrition
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dictyoglomus
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mannans
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food industry
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n-acyl-d-glucosamine
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epimerizes
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isomerizes
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cello-oligosaccharides
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spirochaeta
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pharmacology
- 5.1.3.11
- synthesis
-
epimerization
- lactulose
- caldicellulosiruptor
- saccharolyticus
-
isomerization
- d-glucose
- ruminococcus
- albus
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prebiotic
- d-mannose
- marinus
- turgidum
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rhodothermus
- nutrition
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dictyoglomus
- mannans
- food industry
- n-acyl-d-glucosamine
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epimerizes
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isomerizes
- cello-oligosaccharides
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spirochaeta
- pharmacology
Reaction
Synonyms
B15CE, BfCE, BN699_02313, Caob-CE, CE-NE1, ce13, cellobiose 2-epimerase, CS-HRCE, Csac_0294, CsCE, CvCE, DfCE, Dfer_1975, DsCE, DtCE, Dtur_0652, EcCE, EpiA, epilactose-producing cellobiose 2-epimerase, Epimerase, cellobiose, FbCE, FjCE, Fjoh_4956, HaCE, Haur_0887, mannobiose 2-epimerase, md1, md2, PhCE, Phep_3983, RACE, Rmar_2439, RmCE, SdCE, Sde_0508, SlCE, Slin_0271, Spith_0082, StCE, STHERM_c00950, TERTU_4095, Thsa-CE, TtCE
ECTree
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Specific Activity
Specific Activity on EC 5.1.3.11 - cellobiose epimerase
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0.009
substrate: 4-O-alpha-D-glucopyranosyl-D-mannose, pH 7.0, 60°C
0.0095
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in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-fructose as substrate at 75°C
0.035
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in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-glucose as substrate at 75°C
0.04
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in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-xylose as substrate at 75°C
0.06
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in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-lyxose as substrate at 75°C
0.15
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in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-mannose as substrate at 75°C
10.4
purified recombinant enzyme, pH 7.5, 30°C, substrate lactose
10.77
12.06
purified recombinant mutant E161D/N365P, pH 7.5, 80°C, substrate lactose
13.5
substrate lactose, recombinant enzyme, pH 7.0, 60°C
13.68
substrate: lactose, pH 7.5, 80°C, mutant enzyme A12S
14.2
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purified recombinant His-tagged enzyme, substrate lactose, pH 7.0, 70°C
14.3
substrate: 4-O-beta-D-glucopyranosyl-D-mannose, pH 7.0, 60°C
15.48
substrate cellobiose, recombinant enzyme, pH 7.0, 60°C
16.8 - 24.4
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purified recombinant His-tagged enzyme, substrate cellolbiose, pH 7.0, 70°C
17.45
substrate: lactose, pH 7.5, 80°C, mutant enzyme A12S/K328I
19.6
substrate: lactose, pH 7.5, 80°C, mutant enzyme R5M/A12S/F231L/K328I
201
purified recombinant enzyme, pH 7.5, 25°C, substrate beta-1,4-mannobiose
21.1
purified recombinant enzyme, pH 7.5, 30°C, substrate epilactose
25.85
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recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C
28.4
29.8
substrate mannobiose, recombinant enzyme, pH 7.0, 60°C
3
the specific activity toward cellobiose is 35 micromol/min/mg at a pH optimum 7.8 in 100 mM glycylglycine buffer
3.6
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in 50 mM PIPES buffer (pH 7.5) containing 10 mM cellobiose as substrate at 75°C
30
purified recombinnat His-tagged enzyme, substrate lactose, pH 7.5, 80°C
30.08
substrate: lactose, pH 7.5, 80°C, mutant enzyme R5M/A12S/I52V/F231L/K328I
311.34
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C
38.81
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C
46.91
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C
51
58.3
recombinant enzyme after first purification step on a DEAE Sepharose FF column
58.91
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C
6.3
6.89
purified recombinant mutant E161D/S180P/S351G, pH 7.5, 80°C, substrate lactose
61.8
recombinant enzyme after second purification step on a hydroxyapatite column, substrate is cellobiose
62.3
purified recombinant enzyme, pH 7.5, 25°C, substrate cellobiose
7.7
purified recombinant enzyme, pH 7.5, 25°C, substrate epilactose
71.6
purified recombinant enzyme, pH 7.5, 30°C, substrate cellobiose
74.39
purified recombinant enzyme, pH 7.5, 50°C, substrate lactose
76.78
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C
79.7
recombinant enzyme after second purification step on a hydroxyapatite column, substrate is lactose
9.06
purified recombinant enzyme, pH 7.5, 25°C, substrate lactose
90.58
purified recombinant enzyme, pH 7.5, 50°C, substrate lactose
93.6
purified recombinant His6-tagged enzyme, substrate lactose, pH 7.5, 70°C
97.5
purified recombinant enzyme, pH 7.5, 30°C, substrate beta-1,4-mannobiose
additional information
purified recombinant wild-type enzyme, pH 7.5, 80°C, substrate lactose
10.77
substrate: lactose, pH 7.5, 80°C, wild-type enzyme
isomerase and epimerase activities of wild-type and mutant enzymes, overview
additional information
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isomerase and epimerase activities of wild-type and mutant enzymes, overview