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5.1.3.11: cellobiose epimerase

This is an abbreviated version!
For detailed information about cellobiose epimerase, go to the full flat file.

Word Map on EC 5.1.3.11

Reaction

cellobiose
=
4-O-beta-D-glucopyranosyl-D-mannose

Synonyms

B15CE, BfCE, BN699_02313, Caob-CE, CE-NE1, ce13, cellobiose 2-epimerase, CS-HRCE, Csac_0294, CsCE, CvCE, DfCE, Dfer_1975, DsCE, DtCE, Dtur_0652, EcCE, EpiA, epilactose-producing cellobiose 2-epimerase, Epimerase, cellobiose, FbCE, FjCE, Fjoh_4956, HaCE, Haur_0887, mannobiose 2-epimerase, md1, md2, PhCE, Phep_3983, RACE, Rmar_2439, RmCE, SdCE, Sde_0508, SlCE, Slin_0271, Spith_0082, StCE, STHERM_c00950, TERTU_4095, Thsa-CE, TtCE

ECTree

     5 Isomerases
         5.1 Racemases and epimerases
             5.1.3 Acting on carbohydrates and derivatives
                5.1.3.11 cellobiose epimerase

Specific Activity

Specific Activity on EC 5.1.3.11 - cellobiose epimerase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.009
substrate: 4-O-alpha-D-glucopyranosyl-D-mannose, pH 7.0, 60°C
0.0095
-
in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-fructose as substrate at 75°C
0.02
substrate: D-glucose, pH 7.0, 60°C
0.035
-
in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-glucose as substrate at 75°C
0.04
-
in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-xylose as substrate at 75°C
0.05
substrate: D-mannose, pH 7.0, 60°C
0.06
-
in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-lyxose as substrate at 75°C
0.1
substrate: cellotriose, pH 7.0, 60°C
0.15
-
in 50 mM PIPES buffer (pH 7.5) containing 10 mM D-mannose as substrate at 75°C
0.42
substrate: maltotriose, pH 7.0, 60°C
0.44
F114A mutant, kinetic value for cellobiose
0.53
W303F mutant, kinetic value for cellobiose
1.15
F114A mutant, kinetic value for cellobiose
1.4
M378T mutant, kinetic value for cellobiose
10.4
purified recombinant enzyme, pH 7.5, 30°C, substrate lactose
10.77
10.9
M62T mutant, kinetic value for cellobiose
12.06
purified recombinant mutant E161D/N365P, pH 7.5, 80°C, substrate lactose
12.1
R377A mutant, kinetic value for cellobiose
12.3
M95T mutant, kinetic value for cellobiose
13.5
substrate lactose, recombinant enzyme, pH 7.0, 60°C
13.68
substrate: lactose, pH 7.5, 80°C, mutant enzyme A12S
14.2
-
purified recombinant His-tagged enzyme, substrate lactose, pH 7.0, 70°C
14.3
substrate: 4-O-beta-D-glucopyranosyl-D-mannose, pH 7.0, 60°C
15.48
substrate cellobiose, recombinant enzyme, pH 7.0, 60°C
15.5
substrate: lactose, pH 7.0, 60°C
16.8 - 24.4
-
purified recombinant His-tagged enzyme, substrate cellolbiose, pH 7.0, 70°C
17.45
substrate: lactose, pH 7.5, 80°C, mutant enzyme A12S/K328I
19.6
substrate: lactose, pH 7.5, 80°C, mutant enzyme R5M/A12S/F231L/K328I
201
purified recombinant enzyme, pH 7.5, 25°C, substrate beta-1,4-mannobiose
21.1
purified recombinant enzyme, pH 7.5, 30°C, substrate epilactose
21.8
substrate: cellobiose, pH 7.0, 60°C
22.7
W55F mutant, kinetic value for cellobiose
25.5
substrate: mannobiose, pH 7.0, 60°C
25.8
M380T mutant, kinetic value for cellobiose
25.85
-
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C
29.2
substrate: cellobiose, pH 7.0, 60°C
29.8
substrate mannobiose, recombinant enzyme, pH 7.0, 60°C
29.9
crude extract after expression in Escherichia coli cells
3
the specific activity toward cellobiose is 3–5 micromol/min/mg at a pH optimum 7.8 in 100 mM glycylglycine buffer
3.6
-
in 50 mM PIPES buffer (pH 7.5) containing 10 mM cellobiose as substrate at 75°C
30
purified recombinnat His-tagged enzyme, substrate lactose, pH 7.5, 80°C
30.08
substrate: lactose, pH 7.5, 80°C, mutant enzyme R5M/A12S/I52V/F231L/K328I
311.34
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C
38.81
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C
41.9
substrate: mannobiose, pH 7.0, 60°C
42
W55F mutant, kinetic value for cellobiose
46.91
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C
5.6
M106T mutant, kinetic value for cellobiose
5.99
W303F mutant, kinetic value for cellobiose
58.3
recombinant enzyme after first purification step on a DEAE Sepharose FF column
58.91
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C
6.35
M212T mutant, kinetic value for cellobiose
6.6
R377A mutant, kinetic value for cellobiose
6.89
purified recombinant mutant E161D/S180P/S351G, pH 7.5, 80°C, substrate lactose
61.8
recombinant enzyme after second purification step on a hydroxyapatite column, substrate is cellobiose
62.3
purified recombinant enzyme, pH 7.5, 25°C, substrate cellobiose
7.7
purified recombinant enzyme, pH 7.5, 25°C, substrate epilactose
7.8
substrate: epilactose, pH 7.0, 60°C
71.6
purified recombinant enzyme, pH 7.5, 30°C, substrate cellobiose
74.39
purified recombinant enzyme, pH 7.5, 50°C, substrate lactose
76.78
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C
79.7
recombinant enzyme after second purification step on a hydroxyapatite column, substrate is lactose
8.73
M96T mutant, kinetic value for cellobiose
9
M270T mutant, kinetic value for cellobiose
9.06
purified recombinant enzyme, pH 7.5, 25°C, substrate lactose
9.9
substrate: maltose, pH 7.0, 60°C
90.58
purified recombinant enzyme, pH 7.5, 50°C, substrate lactose
93.6
purified recombinant His6-tagged enzyme, substrate lactose, pH 7.5, 70°C
97.5
purified recombinant enzyme, pH 7.5, 30°C, substrate beta-1,4-mannobiose
additional information